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  2. Metabolic Enzyme/Protease Apoptosis
  3. MMP Apoptosis
  4. Edaravone

Edaravone  (Synonyms: 依達拉奉; MCI-186)

目錄號: HY-B0099 純度: 99.89%
COA 產(chǎn)品使用指南

Edaravone 是一種新穎的，有效的自由基清除劑，能夠抑制大鼠與 MMP-9 有關(guān)的腦出血。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Edaravone Chemical Structure

Edaravone Chemical Structure

CAS No. : 89-25-8

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥385
In-stock
5 g ￥350
In-stock
10 g ￥490
In-stock
25 g ￥735
In-stock
50 g   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Edaravone:

    Edaravone purchased from MCE. Usage Cited in: Int Wound J. 2023 Apr 11.  [Abstract]

    Edaravone (EDA; 20.0?mg/kg/day; i.p.; 7 days) significantly reduces the number of apoptotic cells in mice.

    Edaravone purchased from MCE. Usage Cited in: Int Wound J. 2023 Apr 11.  [Abstract]

    Edaravone (EDA; 20.0?mg/kg/day; i.p.; 7 days) significantly increases the protein expression of TGF-β1, VEGF and MMP9 in mice.

    Edaravone purchased from MCE. Usage Cited in: Int Wound J. 2023 Apr 11.  [Abstract]

    Edaravone (EDA; 20.0?mg/kg/day; i.p.; 7 days) significantly promotes VEGF and MMP9 expression in mice.

    Edaravone purchased from MCE. Usage Cited in: Front Physiol. 2020 Jan 15;10:1596.

    TNF-α expression is significantly increased by treating C2C12 cells with 250 μM H2O2. In addition, pretreatment with edaravone at 100 μM, TNF-α expression is significantly suppressed (? compared with 0 μM H2O2, p < 0.01; # compared with 250 μM H2O2, p < 0.01).

    Edaravone purchased from MCE. Usage Cited in: Front Physiol. 2020 Jan 15;10:1596.

    After treating C2C12 cells with 250 μM H2O2, viability is significantly decreased, and improvement in survival rate was observed in the group treated with Edaravone at 100 μM (?compared with 0 μM H2O2, p < 0.01; # compared with 250 μM H2O2, p < 0.01).

    Edaravone purchased from MCE. Usage Cited in: Front Physiol. 2020 Jan 15;10:1596.

    A) HE staining of muscle tissue after artery ligation shows that the cells are round, and reveals infiltration of inflammatory cells in both control (Con) and ob/ob (Ob) mice. (B) MyoD immunostaining is lower in Ob than Con at 7 days after ligation of the femoral artery. However, pretreatment with Edaravone increases the expression of MyoD in obese mice.

    • 生物活性

    • 實驗參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Edaravone is a strong novel free radical scavenger, and inhibits MMP-9-related brain hemorrhage in rats treated with tissue plasminogen activator.

    細胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    HBMEC-2 EC50
    4.61 μM
    Compound: Edaravone
    Neuroprotective activity against H2O2-induced cell damage in human HBMEC2 cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay
    Neuroprotective activity against H2O2-induced cell damage in human HBMEC2 cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay
    		[PMID: 31541868]
    PC-12 EC50
    9.2 μM
    Compound: Edaravone
    Neuroprotective activity against 12.5 mM glutamate-induced toxicity in rat PC12 cells assessed as cell viability after 24 hrs by MTT assay
    Neuroprotective activity against 12.5 mM glutamate-induced toxicity in rat PC12 cells assessed as cell viability after 24 hrs by MTT assay
    		[PMID: 28394604]
    Platelet IC50
    0.25 mM
    Compound: Eda
    Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method
    Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method
    		[PMID: 22827516]
    Platelet IC50
    0.85 mM
    Compound: Eda
    Inhibition of adenosine diphosphate-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method
    Inhibition of adenosine diphosphate-induced platelet aggregation in rabbit platelet rich plasma by Born's turbidimetric method
    		[PMID: 22827516]
    SH-SY5Y EC50
    3.75 μM
    Compound: Edaravone
    Neuroprotective activity against H2O2-induced cell damage in human SH-SY5Y cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay
    Neuroprotective activity against H2O2-induced cell damage in human SH-SY5Y cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay
    		[PMID: 31541868]
    體外研究
    (In Vitro)

    Edaravone 對谷氨酸的毒性具有預防和改善作用。Edaravone 的預處理降低了谷氨酸對 SGN 的毒性。Edaravone 減少由谷氨酸引起的細胞凋亡和壞死。Edaravone (500 μM) 的預處理可將這些變化逆轉(zhuǎn)至接近正常水平。Edaravone 對谷氨酸誘導的SGNs細胞凋亡的保護作用與PI3K/Akt通路和Bcl-2蛋白家族有關(guān)[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    Edaravone 通過抑制內(nèi)皮損傷和改善腦缺血中的神經(jīng)元損傷來發(fā)揮神經(jīng)保護作用。Edaravone 提供了 NOS 的理想特性：它增加了 eNOS (有益于挽救缺血性中風的 NOS) 并減少了 nNOS 和 iNOS (有害的 NOS)。Edaravone 預處理可減少溶栓處理引起的再灌注后腦水腫和出血事件[1]。Edaravone 顯著減少梗死面積，Edaravone 組大鼠的平均梗死面積 (227.6 mm3) 明顯低于對照組 (264.0 mm3) . Edaravone 處理還減少了缺血后出血體積 (Edaravone 處理大鼠為 53.4 mm3，對照組為 176.4 mm3)。此外，Edaravone 處理大鼠的出血體積與梗死體積之比 (23.5%) 低于未處理大鼠 (63.2%)[2]。在 Edaravone (20 mg/kg) 處理的大鼠中，胼胝體、生發(fā)基質(zhì)和大腦皮層的星形膠質(zhì)細胞活性 (膠質(zhì)原纖維酸性蛋白) 和凋亡細胞 (caspase-3) 減少[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    174.20

    Formula

    C10H10N2O
    CAS 號
    性狀

    固體

    顏色

    Off-white to yellow

    中文名稱

    依達拉奉
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    溶解性數(shù)據(jù)
    細胞實驗： 

    DMSO 中的溶解度 : 100 mg/mL (574.05 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 5.7405 mL 28.7026 mL 57.4053 mL
    5 mM 1.1481 mL 5.7405 mL 11.4811 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (14.35 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (14.35 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    請輸入您的動物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計算結(jié)果
    工作液所需濃度 : mg/mL
    儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
    請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.89%

    參考文獻
    Cell Assay
    [4]

    Cell viability is quantified by MTT assay and trypan blue staining. MTT (5 mg/mL, 20 μL) is added to each well and incubated for 4 h at 37°C after the drug treatments. The medium is removed and the cell pellet is dissolved in DMSO. Then, the optical density (OD) values are measured at 570 nm using an ELISA reader.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 5.7405 mL 28.7026 mL 57.4053 mL 143.5132 mL
    5 mM 1.1481 mL 5.7405 mL 11.4811 mL 28.7026 mL
    10 mM 0.5741 mL 2.8703 mL 5.7405 mL 14.3513 mL
    15 mM 0.3827 mL 1.9135 mL 3.8270 mL 9.5675 mL
    20 mM 0.2870 mL 1.4351 mL 2.8703 mL 7.1757 mL
    25 mM 0.2296 mL 1.1481 mL 2.2962 mL 5.7405 mL
    30 mM 0.1914 mL 0.9568 mL 1.9135 mL 4.7838 mL
    40 mM 0.1435 mL 0.7176 mL 1.4351 mL 3.5878 mL
    50 mM 0.1148 mL 0.5741 mL 1.1481 mL 2.8703 mL
    60 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3919 mL
    80 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7939 mL
    100 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4351 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Edaravone
    目錄號：
    HY-B0099
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