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  2. NF-κB
  3. NF-κB
  4. Pyrrolidinedithiocarbamate ammonium

Pyrrolidinedithiocarbamate ammonium  (Synonyms: 吡咯烷二硫代氨基甲酸銨; Ammonium pyrrolidinedithiocarbamate; PDTC ammonium; APDC)

目錄號: HY-18738 純度: ≥98.0%
COA 產(chǎn)品使用指南 技術(shù)支持

Pyrrolidinedithiocarbamate ammonium (Ammonium pyrrolidinedithiocarbamate) 是選擇性和具有血腦屏障通透性的 NF-κB 抑制劑。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Pyrrolidinedithiocarbamate ammonium Chemical Structure

Pyrrolidinedithiocarbamate ammonium Chemical Structure

CAS No. : 5108-96-3

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Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 106 篇科研文獻

WB
RT-PCR
Proliferation Assay
Cell Viability Assay

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Acta Pharmacol Sin. 2023 Feb 24.  [Abstract]

    Pyrrolidinedithiocarbamate ammonium (PDTC; 100 μM; 24 h) decreases viability of HepG2 and Huh7 cells on the basis of aspirin.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638.

    SCT2 depletion-senescent LX2 cells are treated with rIL-1α protein and addressed in NF-κB inhibitor PDTC (100 μM), and the analysis of mRNAs of proinflammatory SASP IL1A, IL1B, IL6 and IL8 by qPCR.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Clin Transl Med. 2022 Jun;12(6):e850.  [Abstract]

    PEMs are treated with the NF-κB inhibitor PDTC (100 μM) for 1h followed by BSA or UGRP1 (0.5 μg/mL) treatment for 6 h, then stimulated by Pam3CSK4 for 0.5 h, p65 in the whole cell lysate and nucleus is analysed by immunoblot.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Clin Transl Med. 2022 Jun;12(6):e850.  [Abstract]

    PEMs are treated with the NF-κB inhibitor PDTC (100 μM) for 1 h followed by BSA or UGRP1 (0.5 μg/mL) treatment and Pam3CSK4 stimulation for 6 h to measure Il6 mRNA by qRT‐PCR .

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: JACC Basic Transl Sci. 2022 Aug 3;7(8):820-840.

    To pharmacologically inhibit NFκB, mice are given Pyrrolidinedithiocarbamate ammonium (PDTC; 100 mg/kg).

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Int J Biol Macromol. 2022 Oct 21;S0141-8130(22)02401-1.

    TLR4 and downstream pathways are subjected to relevant western blotting. After the treatment with TJ-M2010, TRAF-stop, and PDTC (5 μM) for 1 h, DCs are stimulated with 10 μg/mL CARP2 for 0, 45, and 90 min.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oncogene. 2022 Mar;41(13):1866-1881.  [Abstract]

    The Cxcl10 mRNA expression in B16F0 cells treated with IFNα (1 000 U/ml) for 12 hours, in presence of PDTC (10 μM) or BAY 11-7082 (5 μM).

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Theranostics. 2021 Jan 19;11(7):3392-3416.  [Abstract]

    Treatment with NF-κB (PDTC) inhibitor in both PC9 and H1975 cells treated with different concentrations of specific inhibitor for 72 h and Western blotting is performed.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Redox Biol. 2021 Oct;46:102098.

    Several specific pharmacological inhibitors, including Pyrrolidine dithiocarbamate (PDTC, 100 μM), Wortmannin (Wort), Stattic, PD98059 and Bisindolylmaleimide I (Bis), are pre-administered to the cardiomyocytes in the HG + Pae group and then the expression of Opa1 is quantified.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: EMBO Rep. 2021 Jul 5;22(7):e51678.  [Abstract]

    qPCR analysis of Mmps including Mmp9, Mmp10, and Mmp12 from THP‐1 cells stimulated with AG (1 μg/mL) for 24 h in the absence or presence of PDTC at the concentration of 10 μM.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Int J Biol Sci. 2019 Jan 24;15(3):688-700.  [Abstract]

    T24 cells are treated with PDTC at various concentrations for 24-h, and cell viability and IC50 values are measured using MTS assays.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Feb 8;10(2):113.   [Abstract]

    RAW cells are pre-treated with rMMP-9 and/or PDTC (50 μM) 1 h followed by 18 h of PBS/LPS challenged, and then expressions of representative M1 marker genes.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oncoimmunology. 2018 Aug 23;7(11):e1461303.  [Abstract]

    The expression of Oct4 in A549 and H460 cells treated with or without rhIL-17A and these molecular inhibitors (NF-kB inhibitor: PDAC, Notch1 inhibitor: LY411575) are assessed by western blotting.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oncoimmunology. 2018 Aug 23;7(11):e1461303.  [Abstract]

    The expression of Oct4 in A549 and H460 cells treated with or without rhIL-17A and these molecular inhibitors (NF-kB inhibitor: PDAC, Notch1 inhibitor: LY411575) are assessed by western blotting.

    Pyrrolidinedithiocarbamate ammonium purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2017;2017:3869561.  [Abstract]

    N-formyl-Met-Leu-Phe (fMLP; 10?nM) are used to stimulate the cells for 2?min, and the polymorphonuclear neutrophils (PMNs) without fMLP serve as the control. NPS-2143 (100?nM), AMG 073 (100?nM), and PDTC (100?nM) are added and incubated. Protein expression results are representative of three experiments.

    查看 NF-κB 亞型特異性產(chǎn)品:

    • 生物活性

    • 實驗參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Pyrrolidinedithiocarbamate ammonium (Ammonium pyrrolidinedithiocarbamate) is a selective and blood-brain barrier (BBB) permeable NF-κB inhibitor.

    IC50 & Target[1]

    NF-κB

     

    細胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    ACHN GI50
    0.23 μM
    Compound: PDTC
    Cytotoxicity against human ACHN cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    Cytotoxicity against human ACHN cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    		[PMID: 25953156]
    BV-2 IC50
    10.8 μM
    Compound: PDTC
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    		[PMID: 28169537]
    HCT-15 GI50
    0.13 μM
    Compound: PDTC
    Cytotoxicity against human HCT15 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    Cytotoxicity against human HCT15 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    		[PMID: 25953156]
    Jurkat IC50
    5.12 μM
    Compound: Pyrrolidinedithiocarbamate
    Antiinflammatory activity in PMA induced human Jurkat cells assessed as inhibition of IL-2 production by EIA assay
    Antiinflammatory activity in PMA induced human Jurkat cells assessed as inhibition of IL-2 production by EIA assay
    		[PMID: 36496202]
    MDA-MB-231 GI50
    0.16 μM
    Compound: PDTC
    Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    		[PMID: 25953156]
    NCI-H23 GI50
    0.1 μM
    Compound: PDTC
    Cytotoxicity against human NCI-H23 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    Cytotoxicity against human NCI-H23 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    		[PMID: 25953156]
    NUGC-3 GI50
    0.14 μM
    Compound: PDTC
    Cytotoxicity against human NUGC3 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    Cytotoxicity against human NUGC3 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    		[PMID: 25953156]
    PC-3 GI50
    0.17 μM
    Compound: PDTC
    Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by sulforhodamine B assay
    		[PMID: 25953156]
    RAW264.7 IC50
    34.5 μM
    Compound: PDTC
    Inhibition of NF-kappaB (unknown origin) transcriptional activity in LPS-induced mouse RAW264.7 cells after 1 hr by secreted alkaline phosphatase assay
    Inhibition of NF-kappaB (unknown origin) transcriptional activity in LPS-induced mouse RAW264.7 cells after 1 hr by secreted alkaline phosphatase assay
    		[PMID: 25953156]
    RAW264.7 IC50
    37 μM
    Compound: PDTC
    Inhibition of NF-kappaB (unknown origin) expressed in mouse RAW264.7 cells pretreated for 1 hr followed by stimulation with LPS measured after 6 hrs by luciferase reporter gene assay
    Inhibition of NF-kappaB (unknown origin) expressed in mouse RAW264.7 cells pretreated for 1 hr followed by stimulation with LPS measured after 6 hrs by luciferase reporter gene assay
    		[PMID: 26928174]
    RAW264.7 IC50
    37.2 μM
    Compound: PDTC
    Inhibition of LPS-induced NFkappaB (unknown origin) transcriptional activity expressed in mouse RAW264.7 cells incubated for 16 hrs by SEAP based reporter gene assay
    Inhibition of LPS-induced NFkappaB (unknown origin) transcriptional activity expressed in mouse RAW264.7 cells incubated for 16 hrs by SEAP based reporter gene assay
    		[PMID: 27096046]
    RAW264.7 IC50
    37.2 μM
    Compound: PDTC
    Inhibition of transcriptional activity of NF-kappaB (unknown origin) transfected in LPS-stimulated mouse RAW264.7 cells after 16 hrs by secretory alkaline phosphatase reporter gene assay
    Inhibition of transcriptional activity of NF-kappaB (unknown origin) transfected in LPS-stimulated mouse RAW264.7 cells after 16 hrs by secretory alkaline phosphatase reporter gene assay
    		[PMID: 24792464]
    THP-1 IC50
    6.11 μM
    Compound: Pyrrolidinedithiocarbamate
    Antiinflammatory activity in LPS induced human THP-1 cells assessed as inhibition of TNF-alpha production by EIA assay
    Antiinflammatory activity in LPS induced human THP-1 cells assessed as inhibition of TNF-alpha production by EIA assay
    		[PMID: 36496202]
    U-937 IC50
    8.3 μg/mL
    Compound: PDTC
    Inhibition of etoposide-induced caspase 3 induction in human U937 cells after 7 hrs
    Inhibition of etoposide-induced caspase 3 induction in human U937 cells after 7 hrs
    		[PMID: 11908994]
    U-937 IC50
    8.3 μg/mL
    Compound: PDTC
    Inhibition of etoposide-induced apoptosis in human U937 cells assessed as inhibition of caspase 3 induction after 7 hrs
    Inhibition of etoposide-induced apoptosis in human U937 cells assessed as inhibition of caspase 3 induction after 7 hrs
    		[PMID: 11374970]
    體外研究
    (In Vitro)

    Pretreatment of cells with Pyrrolidinedithiocarbamate ammonium (Ammonium pyrrolidinedithiocarbamate; 3-1000 μM) dose-dependently attenuate IL-8 production[1].
    Furthermore, pyrrolidinedithiocarbamate ammonium (100 μM) suppresses the accumulation of IL-8 mRNA[1].
    Pyrrolidinedithiocarbamate ammonium inhibits the activation of NF-κB, because it suppresses both NF-κB DNA binding and NF-κB-dependent transcriptional activity. NF-κB inhibition with pyrrolidinedithiocarbamate ammonium decrease IL-8 production by intestinal epithelial cells[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    The DSS+pyrrolidinedithiocarbamate ammonium-treated groupII exhibits suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels are significantly lower in DSS+pyrrolidinedithiocarbamate ammonium-treated groupII. These findings suggest that suppression of NF-κB activity by pyrrolidinedithiocarbamate ammonium can delay the healing of mucosal tissue defects (erosions or ulcers) arising from inflammation, but that it can strongly suppress the expression of inf-lammatory cytokines (IL-1β and TNF-α), resulting in significant alleviation of colitis. pyrrolidinedithiocarbamate ammonium is useful for the treatment of ulcerative colitis[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    164.29

    Formula

    C5H12N2S2
    CAS 號
    性狀

    固體

    顏色

    White to off-white

    中文名稱

    吡咯烷二硫代甲酸銨鹽；四亞甲基二硫代氨基甲酸銨；1-吡咯烷二硫代羧酸銨鹽；吡咯烷二硫代氨基甲酸銨；1-吡咯烷硫代羧酸銨鹽；吡咯二硫代甲酸銨；四次甲基二硫代氨基甲酸銨；1-吡咯烷羰基二硫酸銨鹽
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性數(shù)據(jù)
    細胞實驗： 

    DMSO 中的溶解度 : 100 mg/mL (608.68 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    H2O 中的溶解度 : 50 mg/mL (304.34 mM; 超聲助溶)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 6.0868 mL 30.4340 mL 60.8680 mL
    5 mM 1.2174 mL 6.0868 mL 12.1736 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (30.43 mM); 澄清溶液

      此方案可獲得 ≥ 5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 50.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (30.43 mM); 澄清溶液

      此方案可獲得 ≥ 5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 50.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

    以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請依序添加每種溶劑： PBS

      Solubility: 24 mg/mL (146.08 mM); 澄清溶液; 超聲助溶

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    計算結(jié)果
    工作液所需濃度 : mg/mL
    該產(chǎn)品水溶性佳，請具體參考實測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    純度 & 產(chǎn)品資料

    純度: ≥98.0%

    參考文獻
    Cell Assay
    [1]

    The human colon cancer cell line HT-29 is obtained and cells are grown in modified McCoy’s 5A medium supplemented with 10% fetal bovine serum. To study the effect of pyrrolidinedithiocarbamate ammonium on IL-8 production, HT-29 cells in 96-well plates are induced with 20 ng/mL of IL-1β for 18 h. Various concentrations (3-1000 μM) of pyrrolidinedithiocarbamate or its vehicle (culture medium) are added to the cells 30 min prior to IL-1β stimulation. The concentration of IL-8 in the supernatant is determined using solid-phase enzyme-linked immunosorbent assay[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration

    Animal Administration: [2]Pyrrolidinedithiocarbamate is administered intraperitoneally to mice at dose levels of 100 and 50 mg/kg. Mice are divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+pyrrolidinedithiocarbamate-treated groupI (low-dose group), and DSS+pyrrolidinedithiocarbamate-treated groupII (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-κB and inflammatory cytokines (IL-1β and TNF-α) in tissue are measured[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻

    Pyrrolidinedithiocarbamate ammonium 相關(guān)分類

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 6.0868 mL 30.4340 mL 60.8680 mL 152.1699 mL
    5 mM 1.2174 mL 6.0868 mL 12.1736 mL 30.4340 mL
    10 mM 0.6087 mL 3.0434 mL 6.0868 mL 15.2170 mL
    15 mM 0.4058 mL 2.0289 mL 4.0579 mL 10.1447 mL
    20 mM 0.3043 mL 1.5217 mL 3.0434 mL 7.6085 mL
    25 mM 0.2435 mL 1.2174 mL 2.4347 mL 6.0868 mL
    30 mM 0.2029 mL 1.0145 mL 2.0289 mL 5.0723 mL
    40 mM 0.1522 mL 0.7608 mL 1.5217 mL 3.8042 mL
    50 mM 0.1217 mL 0.6087 mL 1.2174 mL 3.0434 mL
    60 mM 0.1014 mL 0.5072 mL 1.0145 mL 2.5362 mL
    80 mM 0.0761 mL 0.3804 mL 0.7608 mL 1.9021 mL
    100 mM 0.0609 mL 0.3043 mL 0.6087 mL 1.5217 mL

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Pyrrolidinedithiocarbamate ammonium
    目錄號：
    HY-18738
    需求量：