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  2. Cell Cycle/DNA Damage Autophagy Anti-infection NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Topoisomerase Autophagy Bacterial Reactive Oxygen Species Antibiotic Endogenous Metabolite Parasite
  4. Berberine chloride

Berberine chloride  (Synonyms: 鹽酸小檗堿; Natural Yellow 18 chloride)

目錄號(hào): HY-18258 純度: 99.69%
COA 產(chǎn)品使用指南 技術(shù)支持

Berberine chloride 是一種生物堿，常用作抗生素。Berberine chloride 誘導(dǎo)活性氧 (ROS) 生成并抑制 DNA 拓?fù)洚悩?gòu)酶 (topoisomerase)?？鼓[瘤特性。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Berberine chloride Chemical Structure

Berberine chloride Chemical Structure

CAS No. : 633-65-8

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     可免費(fèi)申領(lǐng)三個(gè)不同產(chǎn)品的試用裝。

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥429
In-stock
50 mg ￥390
In-stock
100 mg ￥660
In-stock
500 mg ￥1320
In-stock
1 g   詢價(jià)  
5 g   詢價(jià)  

* Please select Quantity before adding items.

Customer Review

Other Forms of Berberine chloride:

MCE 顧客使用本產(chǎn)品發(fā)表的 45 篇科研文獻(xiàn)

WB
Proliferation Assay
Cell Viability Assay

    Berberine chloride purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 0.625, 1.25, 2.5, 5, 10 μM; 48 h) inhibits the viability of A431cells in a dose-dependent matter.

    Berberine chloride purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 2.5, 5, 10 μM; 48 h) inhibits EGFR phosphorylation in a dose-dependent matter in A431cells.

    Berberine chloride purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 2.5 μM; 10 days) significantly inhibits NCI-H441 colony formation.

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Berberine chloride is an alkaloid that acts as an antibiotic. Berberine chloride induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Antineoplastic properties[1].

    IC50 & Target

    ROS[1]
    DNA topoisomerase[1]
    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    3T3-L1 IC50
    21.84 μM
    Compound: Berberine
    Cytotoxicity against mouse 3T3L1 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
    Cytotoxicity against mouse 3T3L1 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
    		[PMID: 28818460]
    BHK-21 CC50
    > 100 μM
    Compound: 190
    Cytotoxicity against BHK21 cells in presence of ATP by Celltiter-Glo assay
    Cytotoxicity against BHK21 cells in presence of ATP by Celltiter-Glo assay
    		[PMID: 28689975]
    HeLa IC50
    12.08 μg/mL
    Compound: Berberine
    Cytotoxicity against human HeLa cells measured after 48 hrs by MTT assay
    Cytotoxicity against human HeLa cells measured after 48 hrs by MTT assay
    		[PMID: 31821990]
    HeLa 229 IC50
    42.93 μM
    Compound: Berberine
    Cytotoxicity against human HeLa 229 cells measured after 48 hrs by MTT assay
    Cytotoxicity against human HeLa 229 cells measured after 48 hrs by MTT assay
    		[PMID: 31821990]
    Hep 3B2 IC50
    > 50 μM
    Compound: 1
    Antiproliferative activity against human Hep3B cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Antiproliferative activity against human Hep3B cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 36640595]
    HepG2 IC50
    > 50 μM
    Compound: 1
    Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 36640595]
    HepG2 IC50
    > 50 μM
    Compound: 1
    Antiproliferative activity against cisplatin-resistant human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Antiproliferative activity against cisplatin-resistant human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 36640595]
    HepG2 IC50
    46.9 μg/mL
    Compound: 1; BBR
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    		[PMID: 27561717]
    Huh-7 IC50
    > 50 μM
    Compound: 1
    Antiproliferative activity against human Huh-7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Antiproliferative activity against human Huh-7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 36640595]
    HUVEC IC50
    18.34 μM
    Compound: Berberine
    Cytotoxicity against human HUVEC cells assessed as reduction in cell viability by MTT assay
    Cytotoxicity against human HUVEC cells assessed as reduction in cell viability by MTT assay
    		[PMID: 30660827]
    LX-2 IC50
    > 50 μM
    Compound: 1
    Antiproliferative activity against human LX2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Antiproliferative activity against human LX2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 36640595]
    MDCK CC50
    70.22 μg/mL
    Compound: Ber
    Cytotoxicity against MDCK cells incubated for 48 hrs assessed as reduction in cell viability by SRB assay
    Cytotoxicity against MDCK cells incubated for 48 hrs assessed as reduction in cell viability by SRB assay
    		[PMID: 28958846]
    RAW264.7 IC50
    > 30 μM
    Compound: 6
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay
    		[PMID: 22024033]
    RAW264.7 IC50
    6.9 μM
    Compound: Berberine
    Inhibition of PCSK9 mRNA level in mouse RAW264.7 cells by qRT-PCR assay
    Inhibition of PCSK9 mRNA level in mouse RAW264.7 cells by qRT-PCR assay
    		[PMID: 30698432]
    SK-HEP1 IC50
    > 50 μM
    Compound: 1
    Antiproliferative activity against human SK-HEP1 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Antiproliferative activity against human SK-HEP1 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 36640595]
    SMMC-7721 IC50
    18.34 μM
    Compound: Berberine
    Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay
    Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay
    		[PMID: 30660827]
    SW1990 IC50
    18.34 μM
    Compound: Berberine
    Cytotoxicity against human SW1990 cells assessed as reduction in cell viability by MTT assay
    Cytotoxicity against human SW1990 cells assessed as reduction in cell viability by MTT assay
    		[PMID: 30660827]
    Vero IC50
    71.14 μg/mL
    Compound: Berberine
    Cytotoxicity against African green monkey Vero cells measured after 48 hrs by MTT assay
    Cytotoxicity against African green monkey Vero cells measured after 48 hrs by MTT assay
    		[PMID: 31821990]
    Vero C1008 CC50
    221 μM
    Compound: 49
    Cytotoxicity against African green monkey Vero E6 cells measured up to 24 to 72 hrs by MTT assay
    Cytotoxicity against African green monkey Vero E6 cells measured up to 24 to 72 hrs by MTT assay
    		[PMID: 31549836]
    體外研究
    (In Vitro)

    Berberine (1.25-160 μM；72 hours) 對(duì)四種結(jié)直腸癌細(xì)胞系 LoVo、HCT116、SW480 和 HT-29 的增殖具有潛在的抑制作用[1]。
    Berberine (1.25-160 μM；24-72 小時(shí)) 誘導(dǎo) LoVo 細(xì)胞生長的時(shí)間和劑量依賴性抑制[1]。
    LoVo 細(xì)胞暴露于小檗堿 (10 -80 μM) 24 小時(shí)。通過流式細(xì)胞術(shù)對(duì) 40 μM 小檗堿處理的 LoVo 細(xì)胞進(jìn)行細(xì)胞周期分析顯示細(xì)胞在 G2/M 期積累[1]。
    Berberine (10 -80 μM) 抑制細(xì)胞周期蛋白 B1，24 h 后 cdc2 和 cdc25c 蛋白表達(dá)，尤其是在80.0 μM 劑量下[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Proliferation Assay[1]

    Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
    Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
    Incubation Time: 72 hours
    Result: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

    Cell Proliferation Assay[1]

    Cell Line: Colorectal carcinoma cell lines LoVo
    Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
    Incubation Time: 24, 48, 72 hours
    Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

    Cell Cycle Analysis[1]

    Cell Line: LoVo cells
    Concentration: 0, 10, 20, 40, or 80 μM
    Incubation Time: 24 hours
    Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

    Western Blot Analysis[1]

    Cell Line: LoVo cells
    Concentration: 10, 20, 40, or 80 μM
    Incubation Time: 24 hours
    Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression.
    體內(nèi)研究
    (In Vivo)

    Berberine (10、30 或 50 mg/kg/天；胃腸道強(qiáng)飼法；連續(xù) 10 天) 在體內(nèi)抑制人結(jié)直腸腺癌的生長。胃腸道灌胃劑量為 30 和 50 mg/kg/day 的小檗堿對(duì)人結(jié)直腸腺癌裸鼠異種移植物生長的抑制率分別為 33.1% 和 45.3%[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
    Dosage: 10, 30, or 50 mg/kg/day
    Administration: Gastrointestinal gavage; for 10 consecutive days
    Result: Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.
    Clinical Trial
    分子量

    371.81

    Formula

    C20H18ClNO4
    CAS 號(hào)
    性狀

    固體

    顏色

    Light yellow to yellow

    中文名稱

    鹽酸小檗堿；鹽酸黃連素；鹽酸黃蓮素
    結(jié)構(gòu)分類
    初始來源
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    DMSO 中的溶解度 : 8.33 mg/mL (22.40 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 2.6895 mL 13.4477 mL 26.8955 mL
    5 mM 0.5379 mL 2.6895 mL 5.3791 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
    ——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.25 mg/mL (3.36 mM); 澄清溶液

      此方案可獲得 ≥ 1.25 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 12.5 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.25 mg/mL (3.36 mM); 澄清溶液

      此方案可獲得 ≥ 1.25 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 12.5 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

    以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請(qǐng)依序添加每種溶劑： 0.5% CMC-Na/saline water

      Solubility: 11 mg/mL (29.59 mM); 懸濁液; 超聲助溶

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
    請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國技術(shù)支持聯(lián)系。
    動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
    請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.69%

    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6895 mL 13.4477 mL 26.8955 mL 67.2386 mL
    5 mM 0.5379 mL 2.6895 mL 5.3791 mL 13.4477 mL
    10 mM 0.2690 mL 1.3448 mL 2.6895 mL 6.7239 mL
    15 mM 0.1793 mL 0.8965 mL 1.7930 mL 4.4826 mL
    20 mM 0.1345 mL 0.6724 mL 1.3448 mL 3.3619 mL
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    產(chǎn)品名稱：
    Berberine chloride
    目錄號(hào)：
    HY-18258
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