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  2. Immunology/Inflammation
  3. COX
  4. Paradol

Paradol  (Synonyms: 姜酮酚; [6]-Gingerone; [6]-Paradol)

目錄號: HY-14617 純度: 99.42%
COA 產(chǎn)品使用指南

Paradol 是一種姜科植物中發(fā)現(xiàn)的刺激性酚類物質(zhì)。在小鼠皮膚癌變中，Paradol結(jié)合到環(huán)氧合酶 (COX-2) 活性位點，有效抑制腫瘤發(fā)展。

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Paradol Chemical Structure

Paradol Chemical Structure

CAS No. : 27113-22-0

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥286
In-stock
5 mg ￥260
In-stock
10 mg ￥420
In-stock
25 mg ￥750
In-stock
50 mg ￥1200
In-stock
100 mg ￥1920
In-stock
500 mg ￥4600
In-stock
1 g 現(xiàn)貨 詢價
5 g   詢價  
10 g   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Paradol:

查看 COX 亞型特異性產(chǎn)品:

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Paradol is a pungent phenolic substance found in ginger and other Zingiberaceae plants. Paradol is an effective inhibitor of tumor promotion in mouse skin carcinogenesis, binds to cyclooxygenase (COX)-2 active site.

IC50 & Target[1]

COX-2

 

體外研究
(In Vitro)

Paradol ([6]-paradol) induces apoptosis in an oral squamous carcinoma cell line, KB, in a dose-dependent manner. Paradol induces apoptosis through a caspase-3-dependent mechanism[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

Administration of Paradol (6-paradol) (10 mg/kg) clearly reduces the number of Iba1-positive cells 1 and 3 days after the challenge. Moreover, Paradol dramatically reduces the number of Iba1-postive cells in periischemic regions even after 3 days following M/R challenge[3]. Paradol (6-paradol) exhibits the strongest anti-inflammatory effect of several paradol compounds in lipopolysaccharide-stimulated BV2 microglia derived from a mouse brain, including 2-, 4-, 6-, 8-, and 10-paradol. Furthermore, Paradol shows the strongest pungency of all of the known paradol analogues. Paradol also shows the highest contact time at the antiobesity site of action on the basis of the results shown for the absorption of the metabolites in this study[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

278.39

Formula

C17H26O3
CAS 號
性狀

液體（密度：1.0690 g/cm3）

顏色

Light yellow to yellow

中文名稱

姜酮酚；[6]-姜酮酚
結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : ≥ 140 mg/mL (502.89 mM; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.5921 mL 17.9604 mL 35.9208 mL
5 mM 0.7184 mL 3.5921 mL 7.1842 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.98 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.98 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    20% SBE-β-CD in Saline 的配制（4°C，儲存一周）：2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.42%

參考文獻
Cell Assay
[2]

KB, human oral epidermoid carcinoma cell lines (ATCC CCL-17) are plated at a density of 5×103 cells/200 μL/well into 96-well plate. After an overnight growth, the cells are treated with a series of paradol derivatives. All of the derivatives of paradol tested are dissolved in DMSO. The final concentration of DMSO in the culture medium is kept below 0.1% and the controls are treated with DMSO alone. Cell viability is assessed using MTT assay. In brief, after the cells are grown in the media in the absence or presence of the test compounds (e.g., Paradol, 10, 50, 100, 150, and 200 μM) for 48 h, they are then replaced to a 200 μL culture medium containing 0.5 mg/mL MTT for 3 h. The resulting MTT-formazan product is dissolved by an addition of the same volume of DMSO. The amount of formazan is determined by measuring the absorbance at 570 nm[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[3][4]

Mice[3]
Male ICR mice (7 weeks old, 36±2 g) challenged with middle cerebral artery occlusion (MCAO)/reperfusion (M/R) are randomly divided into vehicle (10% Tween80)- or Paradol-administered groups (n=6~7 per group). Paradol dissolved in 10% Tween80 is orally administered (10 mg/kg) into mice at 1, 5, or 10 mg/kg immediately after reperfusion.
Rats[4]
Five-week-old Sprague-Dawley rats (male) are used. At 8 weeks of age, the rats are fasted for 14 h prior to the oral administration of olive oil (1 mL) containing zingerone or 6-, 8-, or 12-paradol (10 mg/kg). Three rats in each group are anesthetized with isoflurane, and samples (0.3 mL) of their blood are collected from their jugular vein using a heparinized needle and syringe at 0 (i.e., prior to the oral administration), 0.25, 0.5, 1, 3, 6, and 24 h after the oral administration of the olive oil containing test compounds. The AUC0-24h values determined using this time schedule are very similar compared with AUC0-24h that sampled the time points more minutely with other materials in our laboratory.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5921 mL 17.9604 mL 35.9208 mL 89.8021 mL
5 mM 0.7184 mL 3.5921 mL 7.1842 mL 17.9604 mL
10 mM 0.3592 mL 1.7960 mL 3.5921 mL 8.9802 mL
15 mM 0.2395 mL 1.1974 mL 2.3947 mL 5.9868 mL
20 mM 0.1796 mL 0.8980 mL 1.7960 mL 4.4901 mL
25 mM 0.1437 mL 0.7184 mL 1.4368 mL 3.5921 mL
30 mM 0.1197 mL 0.5987 mL 1.1974 mL 2.9934 mL
40 mM 0.0898 mL 0.4490 mL 0.8980 mL 2.2451 mL
50 mM 0.0718 mL 0.3592 mL 0.7184 mL 1.7960 mL
60 mM 0.0599 mL 0.2993 mL 0.5987 mL 1.4967 mL
80 mM 0.0449 mL 0.2245 mL 0.4490 mL 1.1225 mL
100 mM 0.0359 mL 0.1796 mL 0.3592 mL 0.8980 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Paradol
目錄號：
HY-14617
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