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  2. Metabolic Enzyme/Protease MAPK/ERK Pathway
  3. Endogenous Metabolite p38 MAPK
  4. 7-Ketocholesterol

7-Ketocholesterol 是膽固醇氧化產(chǎn)物，廣泛存在于動脈粥樣硬化斑塊中，比膽固醇具有更強(qiáng)的致動脈粥樣硬化作用。7-Ketocholesterol 能夠抑制膽汁酸和膽固醇的合成限速酶，如膽固醇 7α-羥化酶和 HMG-CoA 還原酶。7-Ketocholesterol 在體內(nèi)外具有促炎作用，可誘導(dǎo)細(xì)胞凋亡 (apoptosis)。

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7-Ketocholesterol Chemical Structure

7-Ketocholesterol Chemical Structure

CAS No. : 566-28-9

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥705
In-stock
1 mg ￥304
In-stock
5 mg ￥800
In-stock
10 mg ￥1300
In-stock
25 mg ￥2600
In-stock
50 mg ￥3988
In-stock
100 mg ￥5725
In-stock
200 mg   詢價(jià)  
500 mg   詢價(jià)  

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Customer Review

Other Forms of 7-Ketocholesterol:

MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻(xiàn)

查看 p38 MAPK 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

7-Ketocholesterol is an oxidation product of cholesterol, widely present in atherosclerotic plaques, and has a stronger atherogenic effect than cholesterol. 7-Ketocholesterol can inhibit the rate-limiting enzymes involved in bile acid and cholesterol synthesis, such as cholesterol 7α-hydroxylase and HMG-CoA reductase. 7-Ketocholesterol exhibits pro-inflammatory effects both in vivo and in vitro and can induce cell apoptosis (apoptosis)[1].

IC50 & Target

Human Endogenous Metabolite

 

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
ARPE-19 IC50
32.3 μM
Compound: 4
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
C6 IC50
20 μM
Compound: 7KC
Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method
Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method
[PMID: 24211631]
HEK293 EC50
8.7 μM
Compound: 9
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
[PMID: 24928400]
HEK293 EC50
9.7 μM
Compound: 6
Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis
Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis
[PMID: 23830695]
HT-29 IC50
25.7 μM
Compound: 4
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
HT-29 IC50
25.7 μM
Compound: 6
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
Vero IC50
75 μM
Compound: 1
Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay
Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay
[PMID: 24084159]
體外研究
(In Vitro)

7-Ketocholesterol (50 μM，2.5-2 小時(shí)) 可通過 p38 MAPK 通路增強(qiáng)白細(xì)胞與內(nèi)皮細(xì)胞的粘附[4]。
7-Ketocholesterol (2.5-40 μg/mL，24 小時(shí)) 以劑量依賴性方式激活人微血管內(nèi)皮細(xì)胞中的 caspase-3/7、-8 和 -12，從而誘導(dǎo)細(xì)胞凋亡[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[4]

Cell Line: Human umbilical vein endothelial cells (HUVECs)
Concentration: 50 μM
Incubation Time: 0.5-2 h
Result: Stimulated the phosphorylation of p38 MAPK, reaching a peak within 30-120 minutes, with no significant effect on JNK.
Enhanced the adhesion of THP-1 cells to HUVECs and upregulated the expression of E-selectin.
Promoted the expression of E-selectin by activating the transcription factor ATF-2.

Cell Viability Assay[5]

Cell Line: Human umbilical vein endothelial cells (HUVECs)
Concentration: 2.5, 5, 10, 15, 20, 40 μg/mL
Incubation Time: 24 h
Result: The average cell viability of HMVEC cells treated with 7-Ketocholesterol at concentrations of 40, 20, 10, 5, and 2.5 μg/ml for 24 hours was 45.33%, 72.83% (p < 0.001), 82.7% (p < 0.05), 88.47%, and 88.5% (p > 0.05), respectively, while the untreated control was 88.83%.
體內(nèi)研究
(In Vivo)

7-Ketocholesterol (5-20 mg/mL，通過生物降解晶片植入大鼠眼前房，單次給藥) 在棕色挪威大鼠體內(nèi)具有促血管生成和促炎作用[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Brown Norway rats (150-200 g)[3]
Dosage: 5, 10, 15, 20 mg/mL
Administration: Implanting biodegradable wafers containing 7-Ketocholesterol into the anterior chamber of rats' eyes, single dose
Result: Significantly increased angiogenesis, with a large infiltration of macrophages detected by immunohistochemistry.
Promoted the growth of new blood vessels originating from the limbus and extending through the cornea, peaking between days 7 and 10 after implantation.
Led to a marked increase in vascular endothelial growth factor (VEGF), IL-1b, and GRO/KC in the aqueous humor.
分子量

400.64

Formula

C27H44O2
CAS 號
性狀

固體

顏色

White to off-white

結(jié)構(gòu)分類
初始來源
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 6.25 mg/mL (15.60 mM; 超聲助溶 (<60°C); 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.4960 mL 12.4800 mL 24.9601 mL
5 mM 0.4992 mL 2.4960 mL 4.9920 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計(jì)算器
請輸入動物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料

純度: 99.87%

參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4960 mL 12.4800 mL 24.9601 mL 62.4002 mL
5 mM 0.4992 mL 2.4960 mL 4.9920 mL 12.4800 mL
10 mM 0.2496 mL 1.2480 mL 2.4960 mL 6.2400 mL
15 mM 0.1664 mL 0.8320 mL 1.6640 mL 4.1600 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
7-Ketocholesterol
目錄號：
HY-113342
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