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  3. Fluorescent Dye
  4. DiBAC4(3)

DiBAC4(3) 是一種電壓敏感的熒光染料 (λex=490 nm，λem=505 nm)。

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DiBAC4(3) Chemical Structure

DiBAC4(3) Chemical Structure

CAS No. : 70363-83-6

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥796
In-stock
1 mg ￥280
In-stock
5 mg ￥700
In-stock
10 mg ￥1050
In-stock
25 mg ￥2100
In-stock
50 mg   詢價(jià)  
100 mg   詢價(jià)  

* Please select Quantity before adding items.

  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm).

體外研究
(In Vitro)

The membrane hyperpolarization induced by 10 μM Evans blue (EB) in HEKBKα is clearly observed with DiBAC4(3), while the change in membrane potential (MP) by addition of 3 mM tetraethylammonium chloride (TEA) appears more slowly than that measured with microelectrode. The time to peak of hyperpolarization is 2.3±0.9 s (n=4) and 35.0±2.6 s (n=12, P<0.01) by the measurements with microelectrodes and DiBAC4(3), respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

516.63

Formula

C27H40N4O6
CAS 號(hào)
性狀

固體

顏色

Pink to red

Emission (Em)

505
Excitation (Ex)

490
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 50 mg/mL (96.78 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 1.9356 mL 9.6781 mL 19.3562 mL
5 mM 0.3871 mL 1.9356 mL 3.8712 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料

純度: 98.89%

參考文獻(xiàn)
Cell Assay
[1]

Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9356 mL 9.6781 mL 19.3562 mL 48.3905 mL
5 mM 0.3871 mL 1.9356 mL 3.8712 mL 9.6781 mL
10 mM 0.1936 mL 0.9678 mL 1.9356 mL 4.8391 mL
15 mM 0.1290 mL 0.6452 mL 1.2904 mL 3.2260 mL
20 mM 0.0968 mL 0.4839 mL 0.9678 mL 2.4195 mL
25 mM 0.0774 mL 0.3871 mL 0.7742 mL 1.9356 mL
30 mM 0.0645 mL 0.3226 mL 0.6452 mL 1.6130 mL
40 mM 0.0484 mL 0.2420 mL 0.4839 mL 1.2098 mL
50 mM 0.0387 mL 0.1936 mL 0.3871 mL 0.9678 mL
60 mM 0.0323 mL 0.1613 mL 0.3226 mL 0.8065 mL
80 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6049 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
DiBAC4(3)
目錄號(hào)：
HY-101892
需求量：