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  4. ADHP

ADHP  (Synonyms: 10-Acetyl-3,7-dihydroxyphenoxazine)

目錄號: HY-101880 純度: 99.67%
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ADHP 是一種熒光過氧化物酶底物（λex=530 nm, λem=590 nm）。

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ADHP Chemical Structure

ADHP Chemical Structure

CAS No. : 119171-73-2

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3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
1 mg ￥300
In-stock
5 mg ￥850
In-stock
10 mg ￥1318
In-stock
25 mg ￥2163
In-stock
50 mg ￥3225
In-stock
100 mg ￥4738
In-stock
200 mg   詢價  
500 mg   詢價  

* Please select Quantity before adding items.

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

體外研究
(In Vitro)

To obtain the parameters Km and kcat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6 s 1.The kobs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

257.24

Formula

C14H11NO4
CAS 號
性狀

固體

顏色

Light brown to brown

Emission (Em)

585
Excitation (Ex)

571
中文名稱

10-乙?；?3,7-二羥基吩嗪
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細(xì)胞實驗： 

DMSO 中的溶解度 : 125 mg/mL (485.93 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.8874 mL 19.4371 mL 38.8742 mL
5 mM 0.7775 mL 3.8874 mL 7.7748 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

掃碼獲得
動物溶解方案

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
純度 & 產(chǎn)品資料

純度: 99.67%

參考文獻(xiàn)
Kinase Assay
[1]

ADHP, 4-ABAH, 2-ABAH, 4-BAH, 4-FBAH, 4-NBAH, 4-TFMBAH, 3-DMABAH, NaN3 and isoniazid are dissolved in DMSO and subsequently diluted into assay buffer. The final concentration of DMSO in the reaction is less than 0.5 % (v/v), which does not affect fluorescence of the oxidized ADHP product 7-hydroxyl-3H-phenoxazin-3-one (resorufin). Reactions of ADHP (20 μM) are incubated with MPO (2.8 nm) in assay buffer and initiated by the addition of 1/10th volume H2O2 from a serial dilution basin. To determine the effect that the simplest benzoic acid hydrazide inhibitor or its analog 4-TFMBAH has on the heme catalytic ability of MPO, MPO (1.2 μM) is incubated for 10 min with different concentrations of BAH inhibitor (0, 0.025, 0.25, 2.5, 12.5 and 25 mM) with ADHP (40 μM) and timing of the reaction is measured following addition of H2O2 (20 μM) ADHP. All reactions are measured in assay buffer at room temperature. Samples of 20 μL are added to non-reducing sample loading buffers, and then loaded without prior heating and resolved by 4-15% gradient SDS-polyacrylamide gel electrophoresis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8874 mL 19.4371 mL 38.8742 mL 97.1855 mL
5 mM 0.7775 mL 3.8874 mL 7.7748 mL 19.4371 mL
10 mM 0.3887 mL 1.9437 mL 3.8874 mL 9.7186 mL
15 mM 0.2592 mL 1.2958 mL 2.5916 mL 6.4790 mL
20 mM 0.1944 mL 0.9719 mL 1.9437 mL 4.8593 mL
25 mM 0.1555 mL 0.7775 mL 1.5550 mL 3.8874 mL
30 mM 0.1296 mL 0.6479 mL 1.2958 mL 3.2395 mL
40 mM 0.0972 mL 0.4859 mL 0.9719 mL 2.4296 mL
50 mM 0.0777 mL 0.3887 mL 0.7775 mL 1.9437 mL
60 mM 0.0648 mL 0.3240 mL 0.6479 mL 1.6198 mL
80 mM 0.0486 mL 0.2430 mL 0.4859 mL 1.2148 mL
100 mM 0.0389 mL 0.1944 mL 0.3887 mL 0.9719 mL

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產(chǎn)品名稱：
ADHP
目錄號：
HY-101880
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