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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
Human bone marrow cells
Preparation method
The solubility of this compound in DMSO is ≥18.9 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
230.0mg/l, 5.0mg/l
Applications
Aztreonam significantly inhibited human colony forming unit unit-erythroid (cfu-e), forming unit-erythroid (bfu-e) and colony forming units-granulocyte macrophages (cfu-gm) at peak and trough serum concentrations.
Animal models
Male cynomolgus monkeys
Dosage form
40,150 and 300 mg/kg(i.v.), once daily for 4 weeks
Application
Treatment of cynomolgus monkeys with aztreonam at a dose of 40 to 300 mg / kg for 4 weeks resulted in a significant reduction in liver microsomal P450 content. On the other hand, after treatment with aztreonam, no significant changes in liver cytochrome b5 content and NADPH-cytochrome c (P450) reductase activity were observed. After treatment of cynomolgus monkeys with aztreonam, the activity of testosterone 6β-hydroxylase in liver microsomes decreased, but the activity of 2β- and 16α-hydroxylase did not decrease. Aztreonam reduced P450 CMLc in liver microsomes of cynomolgus monkeys.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Charak BS, Louie R, Malloy B, Twomey P, Mazumder A. The effect of amphotericin B, aztreonam, imipenem and cephalosporins on the bone marrow progenitor cell activity. J Antimicrob Chemother. 1991 Jan;27(1):95-104. PubMed PMID: 2050599.
[2]. Ohmori S, Horie T, Hayashi S, Kitada M. Aztreonam decreases hepatic microsomal cytochrome P450 in cynomolgus monkeys. Pharmacology. 1994 Mar;48(3):137-42. PubMed PMID: 8153141.