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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
human hepatoma cell lines (HepG2 cells)
Preparation method
General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
0.5, 2.5, 5, 7.5, 10, 15 μg/ml, 24 h
Applications
Berberine as a new upregulator of liver LDLR (low-density lipoprotein receptor) expression. In HepG2 cells, the effect of berberine was also dose dependent. Northern blot showed a 50% increase in LDLR mRNA in cells treated with 2.5 μg/ml of berberine and a maximal increase of four fold of control was seen with a concentration of 15 μg/ml. The effect of BBR on LDLR was further confirmed in another human hepatoma cell line, Bel-7402. BBR at 2.5 μg/ml increased the LDLR mRNA in these cells by 2.3-fold.
Animal models
female golden hamsters
Dosage form
orally twice a day at 50 mg/kg/d or 100 mg/kg/d for 10 d.
Application
In hamsters, treatment of these hyperlipidemic animals with berberine by oral administration for 10 d resulted in dose-dependent decreases in both serum total cholesterol and LDL-c. After the 10-d treatment, berberine at a dose of 50 mg/kg/d reduced LDL-c by 26%, and at a dose of 100 mg/kg/d, reduced LDL-c by 42% as compared to the control animals on the same HFHC (high-fat and high-cholesterol) diet. The berberine effect was also time dependent. LDLR mRNA and protein levels were elevated in all berberine -treated hamsters in a dose-dependent manner. A 3.5-fold increase in mRNA and a 2.6-fold increase in protein in hamster livers treated with 100 mg/kg/d of berberine were detected.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Kong, W.,Wei, J.,Abidi, P., et al. Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins. Nature Medicine 10(12), 1344-1351 (2004).
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