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D. Vieira ; J. Barralet ; E. J. Harvey , et al. Biosensors,2022,12(10):884. DOI: 10.3390/bios12100884 PubMed ID: 36291022
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Abstract: Matrix metalloproteinases (MMPs) are essential proteins acting directly in the breakdown of the extra cellular matrix and so in cancer invasion and metastasis. Given its impact on tumor angiogenesis, monitoring MMP-14 provides strategic insights on cancer severity and treatment. In this work, we report a new approach to improve the electrochemical interaction of the MMP-14 with the electrode surface while preserving high specificity. This is based on the detection of the hemopexin (PEX) domain of MMP-14, which has a greater availability with a stable and low-cost commercial molecule, as a recognition element. This molecule, called NSC-405020, is specific of the PEX domain of MMP-14 within the binding pocket. Through the covalent grafting of the NSC-405020 molecule on carbon nanotubes (CNTs), we were able to detect and quantify MMP-14 using electrochemical impedance spectroscopy with a linear range of detection of 10 ng?mL?1 to 100 ng?mL?1, and LOD of 7.5 ng?mL?1. The specificity of the inhibitory small molecule was validated against the PEX domain of MMP-1. The inhibitor loaded CNTs system showed as a desirable candidate to become an alternative to the conventional recognition bioelements for the detection of MMP-14.
Keywords: MMP-14 ; electrochemical detection ; PEX domain ; CNT ; surface modification ; cancer
Purchased from AmBeed: 7497-07-6
CAS No. : | 7497-07-6 | MDL No. : | MFCD02006898 |
Formula : | C12H15Cl2NO | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | ARDYECYBETXQFD-UHFFFAOYSA-N |
M.W : | 260.16 | Pubchem ID : | 346721 |
Synonyms : |
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Chemical Name : | 3,4-Dichloro-N-(pentan-2-yl)benzamide |
Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P280-P305+P351+P338 | UN#: | N/A |
Hazard Statements: | H302 | Packing Group: | N/A |
GHS Pictogram: |
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