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Fractionating Chitin-Glucan Complex and Coproducts from?Pleurotus Ostreatus?Mushrooms
Muhammad Ayser ; Wafa Tonny ; Isabella Sanchez Hernandez , et al. Waste Biomass Valori.,2023,15,2897-2910. DOI: 10.1007/s12649-023-02364-5
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Abstract: Purpose: A large amount of wasted mushroom stems are accumulated yearly by the mushroom industry. To reduce this waste, we have proposed a fractionation method to isolate several useful coproducts using reusable solvents. Methods: Coproducts were extracted by sequential solvent extraction before producing chitin-glucan complex from Pleurotus ostreatus (oyster) mushrooms. The extracted β-glucans, polyphenols, and proteins were confirmed by 3,5-dinitrosalicylic acid (DNS), Folin-Ciocalteau, and bicinchoninic acid (BCA) assays respectively. Extracted lipids were analyzed by gas chromatography-mass spectrometry (GC–MS). The chitin-glucan complex was characterized by Fourier-transform infrared spectroscopy (FT-IR), high performance liquid chromatography (HPLC), and powder X-ray diffraction (XRD). Results: The extract yield of chitin-glucan complex was 8.3%. The crystallinity index of the extracted chitin-glucan complex was 71.2% when compared to 85% for crustacean chitin. The reduced crystallinity in mushroom chitin was due to the presence of the residual β-glucans. Conclusion: The reported fractionation method uses less solvent and provides a greener alternative to producing chitin-glucan complex when compared to the conventional methods of using a large volume of harsh chemicals harmful to the environment. Further, fractionating several coproducts while producing the chitin-glucan complex will reduce the total processing cost.
Keywords: Chitin ; Chitosan ; Mushrooms ; Vitamins ; Proteins ; β-Glucans
Purchased from AmBeed: 5995-86-8
CAS No. : | 5995-86-8 | MDL No. : | MFCD00149098 |
Formula : | C7H8O6 | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | IUTKPPDDLYYMBE-UHFFFAOYSA-N |
M.W : | 188.13 | Pubchem ID : | 24721416 |
Synonyms : |
3,4,5-Trihydroxybenzoic acid hydrate
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Chemical Name : | 3,4,5-Trihydroxybenzoic acid hydrate |
Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P261-P305+P351+P338 | UN#: | N/A |
Hazard Statements: | H315-H319-H335 | Packing Group: | N/A |
GHS Pictogram: |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Yield | Reaction Conditions | Operation in experiment |
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In water; at 60℃; for 1h; | (1) Accurately weigh 247 mg of <strong>[19387-91-8]tinidazole</strong> and 376 mg of gallic acid monohydrate in a 50 mL round bottom flaskAdd 20 mL of distilled water and install a reflux unit.(2) Stir the reaction in a water bath at 60 C for 1 hour.The solution gradually changed from a colorless solution to a pale yellow solution.liquid.(3) The reaction solution was filtered through a medium speed filter paper, and the filtrate was placed in a 50 mL beaker, and the beaker mouth was covered with a plastic wrap.Three small mouths were placed with a syringe and allowed to stand for crystallization. The pale yellow powdery solid precipitated after about 24 hours and was a <strong>[19387-91-8]tinidazole</strong>-gallic acid drug cocrystal. |