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CAS No. : | 27200-12-0 | MDL No. : | MFCD00189451 |
Formula : | C15H12O8 | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | KJXSIXMJHKAJOD-LSDHHAIUSA-N |
M.W : | 320.25 | Pubchem ID : | 161557 |
Synonyms : |
Ampelopsin;Ampeloptin;Ampelopsin, Ampeloptin, DHM, Dihydromyricetin;(+)-Dihydromyricetin
|
Chemical Name : | (2R,3R)-3,5,7-Trihydroxy-2-(3,4,5-trihydroxyphenyl)chroman-4-one |
Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P261-P305+P351+P338 | UN#: | N/A |
Hazard Statements: | H302-H315-H319-H335 | Packing Group: | N/A |
GHS Pictogram: |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With tea polyphenol oxidase; In aq. phosphate buffer; at 30℃; for 0.75h;pH 3.5;Enzymatic reaction; | Weigh 75g of dihydromyricetin and epicatechin gallate, respectively.Dissolved in 15L of citrate-dibasic phosphate buffer at pH 3.5,Add a certain amount of tea polyphenol oxidase (addition amount is 2g/L),The ventilation was 30L/min and the reaction was carried out at 30 C for 45 minutes.Reheating quickly raises the temperature to 100 C to inactivate the polyphenol oxidase for 5 minutes.Stop the reaction. The obtained reaction liquid was subjected to column chromatography on a polyamide resin.The column torus diameter ratio is 1:8, the ratio of sample to column volume is 1:5, and the loading rate is 2BV/h.Elution with water, 5% aqueous ethanol solution, 95% aqueous ethanol solution,The 95% ethanol eluting site was collected, concentrated and dried under reduced pressure at 40 C to obtain a crude extract.Further separation and purification using BUCHI medium pressure preparation, column chromatography specifications:15mm*310mm, packing C18, flow rate 3mL/min,The crude extract was dissolved in 25% aqueous ethanol (concentration of 80 mg/mL)As a sample solution, the elution gradient was eluted with 15%-25% aqueous ethanol solution for 3 h.25% ethanol aqueous solution was eluted for 2 h, 25%-60% ethanol aqueous solution was eluted for 3 h,The solution was eluted with 60%-95% aqueous ethanol for 2 h, and a 25% ethanol water elution solution was collected.Concentrate under reduced pressure at 45 C to an alcohol-free taste, and freeze-dry to give Compound 2 (HPLC purity ? 98%). |