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Multifaceted regulation of Sirtuin 2 (Sirt2) Deacetylase Activity
Abeywardana, Maheeshi Yapa ; Whedon, Samuel D ; Lee, Kwangwoon , et al. JBC,2024,107722. DOI: 10.1016/j.jbc.2024.107722 PubMed ID: 39214297
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Abstract: Sirtuin 2 (Sirt2) is a member of the sirtuin family of NAD-dependent lysine deacylases and plays important roles in regulation of the cell cycle and gene expression. As a nucleocytoplasmic deacetylase, Sirt2 has been shown to target both histone and non-histone acetylated protein substrates. The central catalytic domain of Sirt2 is flanked by flexible N- and C-termini, which vary in length and composition with alternative splicing. These termini are further subject to posttranslational modifications (PTMs) including phosphorylation. Here we investigate the function of the N- and C-termini on deacetylation of nuclear substrates by Sirt2. Remarkably, we find that the C-terminus autoinhibits deacetylation, while the N-terminus enhances deacetylation of proteins and peptides, but not nucleosomes—a chromatin model substrate. Using protein semisynthesis we characterize the effect of cell cycle-linked N-terminal phosphorylation at two major phosphorylation sites (Ser23/Ser25) and find that these further enhance protein/peptide deacetylation, with no effect on nucleosome deacetylation. Additionally, we find that VRK1, an established binding partner of both Sirt2 and nucleosomes, can stimulate deacetylation of nucleosomes by Sirt2, likely through an electrostatic mechanism. Taken together, these findings reveal multiple mechanisms regulating the activity of Sirt2, which allow for a broad range of activities across its multiple biological roles.
Purchased from AmBeed: 158171-14-3 ; 166108-71-0
CAS No. : | 158171-14-3 | MDL No. : | MFCD00797869 |
Formula : | C25H24NO8P | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | ZBPUWGDUVAAWJY-QHCPKHFHSA-N |
M.W : | 497.43 | Pubchem ID : | 11005563 |
Synonyms : |
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Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P261-P305+P351+P338 | UN#: | N/A |
Hazard Statements: | H315-H319-H335 | Packing Group: | N/A |
GHS Pictogram: |
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