Wnt-C59 NEW
| Price | $33 | $48 | $75 |
| Package | 2mg | 5mg | 10mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-07 |
Product Details
| Product Name: Wnt-C59 | CAS No.: 1243243-89-1 |
| Purity: 99.91% | Supply Ability: 10g |
| Release date: 2024/11/07 |
Product Introduction
Bioactivity
| 名稱 | Wnt-C59 |
| 描述 | Wnt-C59 (C59) is a highly effective and specific Wnt signaling antagonis with PORCN enzymatic activity. |
| 細胞實驗 | Wnt-C59 (C59) is dissolved in DMSO (10 mM) and stored, and then diluted with appropriate medium before use[1]. Approximately, 1×104 cells are seeded in 24-well plates, and Wnt-C59 (5 μM, 10 μM, and 20 μM) is added the next day. Each group is tested in triplicate and control groups with addition of DMSO are also established. Cell confluence is determined by microscopy at 24, 48, 72, and 96 hours after seeding of cells. The IC50 of Wnt-C59 is determined by MTT assay, using 96-well dishes. Next day, various concentrations of Wnt-C59 are added, and cellular viabilities are measured by a spectrophotometer at both 24 and 48 hours. For sphere formation, approximately one hundred cells are seeded onto the Low Cell Bind Surface 24-well Nunc dish. Each group is done in triplicate and each well had 2 mL medium. Media are changed twice a week, and only half of the media is changed each time. Approximately, 1×103 cells are seeded for each well in the sphere inhibition assay. At 1 to 5 days after plating, all tested cells formed small spheres. Five days later, Wnt-C59 (1 μM, 5 μM, and 20 μM) is added into experimental groups. Abilities for cell growth and sphere images are compared and recorded at the end of the first, second, and third weeks after addition of Wnt-C59, or DMSO in control groups. The sphere growths are observed and recorded daily under microscopy, and the area of spheres is analyzed using Metamorph and recorded as average area (μm2)[1]. |
| 激酶實驗 | Aurora A radioactive Flashplate enzyme assay: Aurora A radioactive Flashplate enzyme assay is conducted to determine the nature and degree of MLN8237-mediated inhibition in vitro. Recombinant Aurora A is expressed in Sf9 cells and purified with GST affinity chromatography. The peptide substrate for Aurora A is conjugated with biotin (Biotin-GLRRASLG). Aurora A kinase (5 nM) is assayed in 50 mM Hepes (pH 7.5), 10 mM MgCl2, 5 mM DTT, 0.05% Tween 20, 2 μM peptide substrate, 3.3 μCi/mL [γ-33P]ATP at 2 μM, and increasing concentrations of MLN8237 by using Image FlashPlates. |
| 體外活性 | In female nude mice with independently transplanted in-situ MMTV-WNT1 tumors, Wnt-C59 (10 mg/kg) effectively reduces the expression of β-catenin-targeted genes and decreases Wnt pathway activity, suppressing tumor cell growth. Additionally, in 14CREER/Rosa-SmoM2 mice, topical administration of Wnt-C59 (5 mg/kg) inhibits cell proliferation. |
| 體內(nèi)活性 | In PORCN-deficient HT1080 cells transfected with PORCN, Wnt-C59 (100 nM) inhibits PORCN activity. Similarly, in HeLa cells transfected with WNT3A-V5, Wnt-C59 (10 -100 nM) suppresses the activity of the PORCN acyltransferase. |
| 存儲條件 | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | Ethanol : 7.6 mg/mL (20 mM) DMSO : 7.6 mg/mL (20 mM) |
| 關(guān)鍵字 | Wnt | WntC59 | Inhibitor | Porcupine | Wnt-C59 | Wnt-C-59 | inhibit | C 59 | C-59 | Wnt C59 |
| 相關(guān)產(chǎn)品 | Urea | PRI-724 | Wnt pathway activator 1 | L-quebrachitol | MSAB | EMT inhibitor-1 | CHIR-99021 | XAV-939 | (E)-Ferulic acid | Nefopam hydrochloride | KY-05009 | Bisdemethoxycurcumin |
| 相關(guān)庫 | 抗結(jié)直腸癌化合物庫 | 成骨分子庫 | 經(jīng)典已知活性庫 | 抑制劑庫 | 神經(jīng)保護化合物庫 | 干細胞分化化合物庫 | 口服活性化合物庫 | 已知活性化合物庫 | Wnt/Hedgehog/Notch 通路化合物庫 | 神經(jīng)元分化化合物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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