WAY-262611 NEW
| Price | $35 | $53 | $105 |
| Package | 5mg | 10mg | 25mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-09 |
Product Details
| Product Name: WAY-262611 | CAS No.: 1123231-07-1 |
| Purity: 99.09% | Supply Ability: 10g |
| Release date: 2024/11/09 |
Product Introduction
Bioactivity
| 名稱 | WAY-262611 |
| 描述 | WAY262611 is a Wnt/β-catenin agonist and an inhibitor of Dkk1. It increases bone formation rate with EC50 of 0.63 μM in TCF-luciferase assay. |
| 激酶實驗 | PI3K and mTOR kinase assay : Enzyme assays are done in fluorescent polarization (FP) format, adapted from the Echelon K-1100 PI3K FP assay kit protocol. Human class I PI3Ks and PI3K-α mutants (E545K and H1047R) are produced in Sf9 or purchased from Upstate Biotech. GST-GRP1 (murine) is produced in Escherichia coli and isolated by GST-Sepharose. Assay buffers are reaction buffer [20 mM HEPES (pH 7.1), 2 mM MgCl2, 0.05% CHAPS, and 0.01% β-mercaptoethanol] and stop/detection buffer [100 mM HEPES (pH 7.5), 4 mM EDTA, 0.05% CHAPS]. FP reaction is run for 30 minutes at room temperature in 20 μL of reaction buffer containing 20 μM phosphatidylinositol 4,5-bisphosphate (PIP2), 25 μM ATP, and <4% DMSO. FP reaction is stopped with 20 μL of stop/detection buffer (10 nM probe and 40 nM GST-GRP), and after 2 hours, data are collected using an Envision plate reader. The routine assays with purified FLAG-TOR (FL and 3.5) are performed in 96-well plates as follows. Enzymes are first diluted in kinase assay buffer (10 mM Hepes (pH 7.4), 50 mM NaCl, 50 mM β-glycerophosphate, 10 mM MnCl2, 0.5 mM DTT, 0.25 μM microcystin LR, and 100 μg/mL BSA). To each well, 12 μL of the diluted enzyme is mixed briefly with 0.5 μL test inhibitor or control vehicle dimethyl sulfoxide (DMSO). The kinase reaction is initiated by adding 12.5 μL kinase assay buffer containing ATP and His6-S6K to give a final reaction volume of 25 μL containing 800 ng/mL FLAG-TOR, 100 μM ATP, and 1.25 μM His6-S6K. The reaction plate is incubated for 2 hours (linear at 1–6 hours) at room temperature with gentle shaking and then terminated by adding 25 μL Stop buffer (20 mM Hepes (pH 7.4), 20 mM EDTA, and 20 mM EGTA). |
| 體外活性 | WAY-262611 demonstrates the highest potency, low kinase inhibition potential, and high solubility in the primary assay [1]. |
| 體內(nèi)活性 | WAY-262611 exhibits exceptional pharmacokinetic properties and, upon oral administration, demonstrates a dose-dependent increase in trabecular bone formation in ovariectomized rats. Calvariae from wild-type mice treated with WAY-262611 display significantly increased bone formation rates (BFR), whereas knockout (KO) animals treated in the same manner show no difference from control, suggesting that WAY-262611 operates through the Wnt β-catenin pathway, likely via inhibition of Dkk-1[1]. |
| 存儲條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 42 mg/mL (131.90 mM) |
| 關(guān)鍵字 | Beta catenin | Inhibitor | β-catenin | WAY 262611 | WAY-262611 | inhibit | WAY262611 |
| 相關(guān)產(chǎn)品 | Urea | PRI-724 | Wnt pathway activator 1 | L-quebrachitol | MSAB | EMT inhibitor-1 | Wogonin | CHIR-99021 | XAV-939 | Nefopam hydrochloride | KY-05009 | Bisdemethoxycurcumin |
| 相關(guān)庫 | 神經(jīng)再生化合物庫 | 經(jīng)典已知活性庫 | 抗癌細胞代謝庫 | 抗卵巢癌化合物庫 | 抑制劑庫 | 神經(jīng)保護化合物庫 | 抗纖維化化合物庫 | 干細胞分化化合物庫 | 已知活性化合物庫 | 細胞骨架化合物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
You may like
Recommended supplier
| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $115.00/1g |
VIP4Y
|
Wuhan Nutra Biotechnology Co.,Ltd
|
2021-05-24 | |
| $1300.00/10gram |
VIP3Y
|
Wuhan Senwayer Century Chemical Co.,Ltd
|
2022-10-31 | |
| $0.00/5mg |
VIP1Y
|
TargetMol Chemicals Inc.
|
2024-10-23 | |
| $0.00/10gram |
VIP3Y
|
Wuhan Senwayer Century Chemical Co.,Ltd
|
2023-05-24 |
United States- Since: 2011-01-07
- Address: 36?Washington?Street, Wellesley?Hills
INQUIRY