成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

Welcome to chemicalbook!
+1 (818) 612-2111
Inquriy
Try our best to find the right business for you.
Do not miss inquiry messages Please log in to view all inquiry messages.

Welcome back!

RFQ
skype
MY Account
Top
Postion:Product Catalog >Biochemical Engineering>Inhibitors>Metabolism>AhR Antagonist>StemRegenin 1
StemRegenin 1
  • StemRegenin 1

StemRegenin 1 NEW

Price $32 $52 $98
Package 5mg 10mg 25mg
Min. Order:
Supply Ability: 10g
Update Time: 2024-11-19

Product Details

Product Name: StemRegenin 1 CAS No.: 1227633-49-9
Purity: 99.78% Supply Ability: 10g
Release date: 2024/11/19

Product Introduction

Bioactivity

NameStemRegenin 1
DescriptionStemRegenin 1 (SR1) is an aryl hydrocarbon receptor (AhR) inhibitor.
Cell ResearchStemRegenin 1 (SR1) is prepared in DMSO and stored, and then diluted with appropriate medium before use[1]. A quantity of 250,000 CB-derived CD34+ cells are cultured with control conditions (DMSO, 0.01%) or StemRegenin 1 (0.75 μM) for 3 weeks. At this point control cultures had expanded 1080-fold and StemRegenin 1 treated cells expanded 2024-fold relative to starting cell numbers. A quantity of 30 to 30,000 uncultured CD34+ CB-derived cells or a fraction of the final culture equivalent to 30 to 10,000 starting cells are transplanted. The cells are injected intravenously via the retro-orbital route into sub-lethally irradiated (300 rads, 200 rads) 6- to 10-week-old NSG mice. Engraftment is performed within 24 h after irradiation. Engraftment is monitored by flow cytometric analysis of blood obtained via retro-orbital sinus or bone marrow using anti-human CD45 and anti-mouse CD45 antibodies. The mice are sacrificed between 13-16 weeks posttransplantation; bone marrow (from both femurs and tibiae), spleen and thymus are collected for analysis. For secondary engraftment, 50% of the bone marrow from each recipient mouse is transplanted into one secondary sub-lethally irradiated NSG mouse. Fifteen weeks after transplantation, bone marrow is harvested from the secondary mice and analyzed by flow cytometry[1].
Kinase AssayKinase assays: PDK1 is assayed in a direct kinase assay and a coupled assay format measuring PDK1- and PtdIns-3,4-P2-mediated activation of AKT2. For the coupled assay, the final assay mixture (60 μL) contained: 15 mM MOPS, pH 7.2, 1 mg/mL bovine serum albumin, 18 mM β-glycerol phosphate, 0.7 mM dithiothreitol, 3 mM EGTA, 10 mM MgOAc, 7.5 μM ATP, 0.2 μCi of [γ-33P]ATP, 7.5 μM biotinylated peptide substrate (biotin-ARRRDGGGAQPFRPRAATF), 0.5 μL of PtdIns-3,4-P2-containing phospholipid vesicles, 60 pg of purified recombinant human PDK1, and 172 ng of purified recombinant human AKT2. After incubation for 2 h at room temperature, the biotin-labeled peptide is captured from 10 μl of the assay mixture on streptavidin-coated SPA beads, and product formation is measured by scintillation proximity in a Wallac MicroBeta counter. The product formed is proportional to the time of incubation and to the amount of PDK1 and inactive AKT2 added. PDK1 is added at suboptimal levels so that the assay could sensitively detect inhibitors of AKT2 activation as well as direct inhibitors of PDK1 or AKT2. To measure PDK1 activity directly, the final assay mixture (60 μL) contained 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1 mM EDTA, 0.1% β-mercaptoethanol, 1 mg/mL bovine serum albumin, 10 mM MgOAc, 10 μM ATP, 0.2 μCi of [γ-33P]ATP, 7.5 μM substrate peptide (H2N-ARRRGVTTKTFCGT), and 60 ng of purified recombinant human PDK1. After 4 h at room temperature, we add 25 mM EDTA and spotted a portion of the reaction mixture on Whatman P81 phosphocellulose paper. The filter paper is washed three times with 0.75% phosphoric acid and once with acetone. After drying, the filter-bound labeled peptide is quantified using a Fuji phosphorimager.
In vitroIn NSG (NOD.Cg-Prkdcscid Il2rgtm1Wj1/SzJ) mice, the proliferation of CB CD34+ cells is enhanced through the activation of SR1, facilitating early and sustained cell engraftment.
In vivoIn vitro, StemRegenin 1 (1 μM) effectively promotes the proliferation of CD34+ cells (EC50 ≈ 120 nM) while concurrently reducing the expression levels of VentX. Furthermore, StemRegenin 1 can inhibit the binding of photoaffinity ligands (PAL) with an IC50 value of 40 nM.
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility InformationH2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 65 mg/mL (151.32 mM), Sonication is recommended.
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
KeywordsStemRegenin-1 | StemRegenin 1 | AhR | StemRegenin1 | inhibit | Inhibitor | Aryl Hydrocarbon Receptor | SR-1 | SR 1
Inhibitors RelatedNimodipine | Carbidopa monohydrate | Diosmin | (-)-Perillaldehyde | Prochloraz | Benzyl butyl phthalate | Skatole | L-Kynurenine | Indole-3-carbinol | Leflunomide | MeBIO | Mexiletine hydrochloride
Related Compound LibrariesTarget-Focused Phenotypic Screening Library | Bioactive Compound Library | Drug Repurposing Compound Library | Inhibitor Library | Stem Cell Differentiation Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Metabolism Disease Compound Library | Human Metabolite Library | Transcription Factor-Targeted Compound Library

Company Profile Introduction

Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers. TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.

You may like

Recommended supplier

Product name Price   Suppliers Update time
$1.00/1g
VIP1Y
Apeloa production Co.,Limited
2024-06-05
$0.00/1BOX
VIP1Y
Shanghai Affida new material science and technology center
2024-05-08
$50.00/1kg
VIP1Y
Hebei Zhuanglai Chemical Trading Co Ltd
2024-12-20
$2.00/1kg
VIP7Y
Career Henan Chemical Co
2018-12-24
$2.00/1kg
VIP7Y
Career Henan Chemical Co
2018-12-24
  • Since: 2011-01-07
  • Address: 36?Washington?Street, Wellesley?Hills
INQUIRY