JSH-23 NEW
| Price | $36 | $52 | $85 |
| Package | 1mg | 2mg | 5mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-17 |
Product Details
| Product Name: JSH-23 | CAS No.: 749886-87-1 |
| Purity: 99.67% | Supply Ability: 10g |
| Release date: 2024/11/17 |
Product Introduction
Bioactivity
| 名稱(chēng) | JSH-23 |
| 描述 | JSH-23 is an NF-κB inhibitor that inhibits NF-κB transcriptional activity (IC50=7.1 μM) but does not affect IκBα degradation. JSH-23 is an antioxidant with anti-inflammatory activity. |
| 細(xì)胞實(shí)驗(yàn) | Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.(Only for Reference) |
| 激酶實(shí)驗(yàn) | Measurement of NF-κB transcriptional activity: Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm. |
| 體外活性 | METHODS: Bone marrow macrophage BMMs were treated with JSH-23 (0.78-200 μM) for 48-96 h. Cell viability was measured by CCK-8 assay. RESULTS: JSH-23 had no detectable toxic effects at concentrations below 50 μM. [1] METHODS: Macrophages RAW 264.7 harboring the reporter gene pNF-κB-SEAP-NPT were treated with LPS (1 μg/mL) and JSH-23 (1-30 μM) for 16 days, and SEAP expression was assayed to reflect NF-κB transcriptional activity. RESULTS: JSH-23 inhibited LPS-induced SEAP expression in a dose-dependent manner by 23±3% at 3 μM, 68±3% at 10 μM, and 103±4% at 30 μM. JSH-23 inhibited NF-κB transcriptional activity. [2] |
| 體內(nèi)活性 | METHODS: To investigate the role in diabetic neuropathy, JSH-23 (1-3 mg/kg) was administered orally to streptozotocin-induced diabetic rats once daily for two weeks. RESULTS: JSH-23 treatment significantly reversed nerve conduction and nerve blood flow deficits in diabetic animals. The treatment also partially corrected the reduced mechanical pain threshold.JSH-23 treatment inhibited nuclear translocation of the p65/p50 subunit in the sciatic nerve. [3] |
| 存儲(chǔ)條件 | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 50 mg/mL (208.04 mM) H2O : < 1 mg/mL (insoluble or slightly soluble) Ethanol : 16 mg/mL (66.6 mM) |
| 關(guān)鍵字 | JSH-23 | Nuclear factor-κB | Nuclear factor-kappaB | NF-κB | Inhibitor | JSH 23 | JSH23 | inhibit |
| 相關(guān)產(chǎn)品 | Sodium Houttuyfonate | Zingerone | Lidocaine | Diethylmaleate | Glucosamine | sodium lauroyl-α-hydroxyethyl sulfonate | Lidocaine hydrochloride | 5-Aminosalicylic Acid | Indole-3-carbinol | Diallyl disulfide | Caffeic Acid Phenethyl Ester | Sodium salicylate |
| 相關(guān)庫(kù) | 鐵死亡化合物庫(kù) | NF-κB 通路分子庫(kù) | 經(jīng)典已知活性庫(kù) | 抗卵巢癌化合物庫(kù) | 抗乳腺癌化合物庫(kù) | 抑制劑庫(kù) | 抗前列腺癌化合物庫(kù) | 抗衰老化合物庫(kù) | 已知活性化合物庫(kù) | 抗肝癌化合物庫(kù) |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
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