ID-8 NEW
| Price | $40 | $56 | $98 |
| Package | 5mg | 10mg | 25mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-19 |
Product Details
| Product Name: ID-8 | CAS No.: 147591-46-6 |
| Purity: 99.32% | Supply Ability: 10g |
| Release date: 2024/11/19 |
Product Introduction
Bioactivity
| Name | ID-8 |
| Description | ID-8, a DYRK inhibitor, sustains embryonic stem cell self-renewal in long-term culture. |
| Cell Research | Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture. (Only for Reference) |
| Kinase Assay | In Vitro Enzymatic Assay for Histone Deacetylases: In vitro activities of the 11 recombinant human zinc-dependent HDAC enzymes are detected by ?uorigenic release of 7-amino-4-methylcoumarin from substrate upon deacetylase enzymatic activity. A series of dilutions of the unique HDAC6 compound, tubacin, and SAHA are prepared with 10% DMSO in HDAC assay buffer, and 5 μL of the dilution was added to a 50-μL reaction so that the ?nal concentration of DMSO is 1% in all of the reactions. The enzymatic reactions are conducted in duplicate at 37 °C for 30 min in a 50-μL mixture containing HDAC assay buffer, 5 μg BSA, an HDAC substrate, an HDAC enzyme, and a test compound. After enzymatic reactions, 50 μL of 2× HDAC developer is added to each well, and the plate is incubated at room temperature for an additional 15 min. Fluorescence intensity is measured at an excitation of 360 nm and an emission of 460 nm using a Synergy microplate reader. Negative (no enzyme, no inhibitor, a drug with no HDAC inhibition activity) and positive controls (known HDAC inhibitor SAHA) are included in the assays. IC50 is determined at the drug concentration that results in 50% reduction of HDAC activity compared with the control. |
| In vivo | ID-8 maintains the expression of the Nanog gene through the activation of Sox2-Oct3/4, enabling the reversible maintenance of ESC (Embryonic Stem Cells) cultures for prolonged periods. In the presence of Wnt, ID-8 modulates the Wnt/β-catenin signaling pathway, enhancing CBP/β-catenin levels to preserve the undifferentiated state of hESCs (human Embryonic Stem Cells), thereby supporting cell proliferation and survival. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 29.8 mg/mL (100 mM) |
| Keywords | cell | embryonic | dual-specificity | DYRK | ESC | Inhibitor | inhibit | Dual specificity tyrosine phosphorylation regulated kinase | stem | regulated | phosphorylation | kinase | tyrosine | ID 8 | ID-8 | Dual specificity tyrosine regulated kinase |
| Inhibitors Related | AZ-Dyrk1B-33 | Harmine | ML 315 | EHT 1610 | ARN25068 | CK2/ERK8-IN-1 | YK-2-69 | AnnH31 | 7BIO | INDY | ML167 | LDN-192960 |
| Related Compound Libraries | Bioactive Compound Library | Tyrosine Kinase Inhibitor Library | Kinase Inhibitor Library | Inhibitor Library | Stem Cell Differentiation Compound Library | NO PAINS Compound Library | Bioactive Compounds Library Max | Cell Cycle Compound Library | Anti-Cancer Compound Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
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