Description of Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside（EPS）from Watson Biotechnology Co., Ltd

Specifications(COA) of Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside（EPS）CAS 96597-16-9

CERTIFICATE OF ANALYSIS

Ethylidene-4-nitrophenyl-α-D-maltoheptaoside is a synthetic carbohydrate compound that serves as a substrate for glycosidase enzymes. It consists of a maltoheptaose (a chain of seven glucose units) linked to a 4-nitrophenyl group, which provides a chromogenic (color-changing) indicator for enzyme activity. The ethylidene group at one end helps stabilize the molecule and makes it more useful in various biochemical assays.

In research, it is often used to study the enzymatic breakdown of carbohydrates, particularly by enzymes like amylases or glucosidases. The release of the 4-nitrophenol moiety upon enzymatic cleavage can be quantitatively measured, allowing scientists to analyze enzyme kinetics and specificity.

Ethylidene-4-nitrophenyl-α-D-maltoheptaoside is typically used in biochemical research, particularly in studies involving glycosidases and carbohydrate-binding proteins. Its applications include:

1. **Enzyme Substrates**: It serves as a substrate for glycosidases, helping researchers study enzyme activity and kinetics.

2. **Carbohydrate Recognition Studies**: This compound can be used to investigate the binding affinities of lectins or other carbohydrate-binding proteins.

3. **Synthesis of Glycosylated Compounds**: It may be utilized in synthesizing complex carbohydrates or glycoproteins for further study.

4. **Analytical Chemistry**: It can be involved in assays to quantify enzyme activity or carbohydrate presence in various samples.

Researchers often use such compounds to better understand the roles and mechanisms of carbohydrates in biological systems.

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