DiI NEW
| Price | $29 |
| Package | 10mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-07 |
Product Details
| Product Name: DiI | CAS No.: 41085-99-8 |
| Purity: 99.95% | Supply Ability: 10g |
| Release date: 2024/11/07 |
Product Introduction
Bioactivity
| 名稱(chēng) | DiI |
| 描述 | DiI (DiIC18(3)) is a lipophilic membrane dye commonly used as a long-term tracer for neurons and other cells. DiI fluoresces very weakly before it enters the cell membrane, and only after it enters the cell membrane can it be excited to emit a strong orange-red fluorescence (λex=549 nm, λem=565 nm). |
| 體外活性 | METHODS: DiI staining: 1. Prepare 1-5 mM of DiI dye stock solution in DMSO or EtOH. Protect from light and store in portions at -20°C. 2. 2. For use, dilute the stock solution with a physiological buffer (e.g., serum-free medium, HBSS, or PBS) to obtain a working solution of 1-5 μM DiI dye. The optimal working concentration must be determined empirically. 3. Suspend cells for staining: Suspend cells at a density of 1×10^6/mL in the dye working solution. Incubate at 37°C for 2-20 min. centrifuge labeled cells at 1000-1500 rpm for 5 min. remove supernatant and resuspend cells in pre-warmed growth medium. Repeat the washing twice. 4. Staining of adherent cells: Cultivate adherent cells on sterile coverslips. Remove the coverslip from the growth medium and gently drain off the excess medium. Pipette 100 μL of staining solution onto one corner of the coverslip and shake gently until all cells are covered. Incubate the coverslips at 37°C for 2-20 min. Drain the dye working solution and wash the coverslips two to three times with growth medium. For each wash, cover the cells with pre-warmed growth medium and incubate for 5-10 min, then drain the medium. 5. The stained cells can be examined by microscopy or flow cytometry. |
| 存儲(chǔ)條件 | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 25 mg/mL (26.77 mM), Sonication and heating to 80℃ are recommended. |
| 關(guān)鍵字 | inhibit | Inhibitor | DiI |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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