BMS-3 NEW
| Price | $45 | $71 | $146 |
| Package | 5mg | 10mg | 25mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-19 |
Product Details
| Product Name: BMS-3 | CAS No.: 1338247-30-5 |
| Purity: 99.31% | Supply Ability: 10g |
| Release date: 2024/11/19 |
Product Introduction
Bioactivity
| Name | BMS-3 |
| Description | BMS-3 is a potent LIMK inhibitor with IC50 values of 5 nM and 6 nM for LIMK1 and LIMK2, respectively. |
| Kinase Assay | The protein kinase domains of human LIMK1 and LIMK2 are expressed as glutathione S-transferase fusion proteins using the Bac-to-Bac system in Sf9 cells. Compounds 1 to 6 (e.g., BMS-3) are assayed for inhibition of LIMK1 and LIMK2 protein kinase activity by radioactive phosphate incorporation into biotinylated full-length human destrin. Reactions are done with a concentration series of compound in 25 mM HEPES, 100 mM NaCl, 5 mM MgCl2, 5 mM MnCl2, 1 μM total ATP, 83 μg/mL biotinylated destrin, 167 ng/mL glutathione S-transferase-LIMK1, or 835 ng/mL glutathione S-transferase-LIMK2 in a total volume of 60 μL at room temperature for 30 min (LIMK1) or 60 min (LIMK2). Reactions are terminated by addition of 140 μL of 20% TCA/100 mM sodium pyrophosphate, and the precipitates are harvested onto GF/C unifilter plates. The radioactivity incorporated is determined using a TopCount after addition of 35 μL Microscint scintillation fluid[1] |
| In vitro | BMS-3 (Compound 2) exhibits a dose-dependent decrease in A549 human lung cancer cell viability by inducing mitotic arrest, characterized by enhanced total nuclear DNA intensity and histone H3 phosphorylation following a 24-hour exposure. It effectively inhibits these cells with an EC50 value of 154 nM[1]. Additionally, BMS-3 elucidates the role of LIMK1 in Cofilin phosphorylation; inhibition of p-LIMK by BMS-3 (1-50 μM) leads to a notable reduction in p-Cofilin levels after a 10-minute incubation under capacitating conditions, with a marked decrease in actin polymerization levels compared to the DMSO controls. Furthermore, under capacitating conditions, mouse sperm exposure to escalating concentrations of BMS-3 (0, 1, 10, and 50 μM) for 90 minutes significantly diminishes the percentage of sperm undergoing acrosomal exocytosis upon Progesterone stimulation[2], highlighting BMS-3's potential in affecting sperm functionality. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 27.5 mg/mL (64.06 mM) |
| Keywords | BMS 3 | inhibit | Inhibitor | LIMKs | BMS-3 | BMS3 | LIM Kinase (LIMK) |
| Inhibitors Related | BMS-5 | CRT-0105950 | LX7101 | TH-257 | SM1-71 | T56-LIMKi | LY2812223 | R-10015 | LIMK-IN-22j | LX-7101 hydrochloride |
| Related Compound Libraries | Target-Focused Phenotypic Screening Library | Bioactive Compound Library | Kinase Inhibitor Library | Inhibitor Library | NO PAINS Compound Library | Bioactive Compounds Library Max | Cell Cycle Compound Library | Anti-Cancer Compound Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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