Description of BIS-Tris Propane from Watson Biotechnology Co., Ltd

Specifications(COA) of BIS-Tris Propane CAS 64431-96-5 Buffering Agent

BIS-Tris Propane, also known as BTP, BTPA, or 1,3-bis[tris(hydroxymethyl)methylamino]propane, is a zwitterionic buffer commonly used in biochemical and molecular biology research. Its chemical structure provides a stable buffering capacity across a wide pH range, particularly useful in experiments requiring precise pH control.

### Applications of BIS-Tris Propane:

1. **Buffering Agent**:

   - **Biological and Biochemical Assays**: BIS-Tris Propane is often used as a buffering agent in various biological and biochemical assays due to its ability to maintain a stable pH over a broad range (pH 6.3 to 9.5). This is particularly useful in enzymatic studies and protein purification processes.

   - **Electrophoresis**: It is used in gel electrophoresis, especially in SDS-PAGE and native PAGE, to stabilize pH during the separation of proteins and nucleic acids.

2. **Chromatography**:

   - **Ion Exchange Chromatography**: BIS-Tris Propane is employed as a buffer in ion exchange chromatography, which is a common technique for separating proteins based on their charge.

3. **Protein Purification**:

   - The compound helps maintain the integrity and activity of proteins during purification processes. It can stabilize proteins against pH-induced denaturation, making it ideal for isolating and studying proteins.

4. **Enzyme Kinetics**:

   - BIS-Tris Propane's buffering properties are valuable in enzyme kinetics studies where maintaining a constant pH is crucial for accurate measurements.

5. **Cell Culture**:

   - It is sometimes used in cell culture media to provide a stable pH environment, which is essential for cell growth and viability.

6. **NMR Studies**:

   - In nuclear magnetic resonance (NMR) spectroscopy, BIS-Tris Propane can be used as a buffer for studying the structure and dynamics of biomolecules.

### Advantages of BIS-Tris Propane:

- **Wide pH Range**: Its effectiveness over a broad pH range makes it versatile for different experimental conditions.

- **Low Metal Binding**: It has low affinity for metal ions, minimizing potential interference with metal-dependent processes.

- **Stability**: BIS-Tris Propane is chemically stable, ensuring consistent buffering capacity during experiments.

Overall, BIS-Tris Propane is a versatile buffer used in a variety of biochemical and biophysical applications, making it an essential component in many laboratory protocols.

Founded in 2006 and headquartered in Wuhan, Hubei, China, Watson Biotechnology Co., Ltd is a leading manufacturing powerhouse specializing in the production of Active Pharmaceutical Ingredients (APIs) and Intermediates, Food and Feed Additives, Agricultural and Aquaculture chemicals, and Fine Chemicals. As a dedicated factory, our operations are driven by a commitment to excellence and innovation, ensuring that our products meet the highest standards of quality and compliance.

With a robust team of over 100 skilled professionals, Watson Biotechnology has established itself as a trusted name in the industry. Our products are meticulously crafted to align with international standards such as USP (United States Pharmacopeia), EP (European Pharmacopoeia), and BP (British Pharmacopoeia), guaranteeing reliability and efficacy.

At Watson Biotechnology, we pride ourselves on our cutting-edge facilities and state-of-the-art technology, which enable us to deliver superior products that cater to a diverse range of needs across various sectors. Our dedication to continuous improvement and customer satisfaction drives us to maintain stringent quality control processes and foster an environment of innovation and growth.

Watson Biotechnology Co., Ltd is a professional manufacturer which provide BIS-Tris Propane with good quality and best price, welcome to contact us to get COA/TDS/MSDS of BIS-Tris Propane.