ANA-12 NEW
| Price | $34 | $47 | $72 |
| Package | 1mg | 2mg | 5mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-07 |
Product Details
| Product Name: ANA-12 | CAS No.: 219766-25-3 |
| Purity: 99.87% | Supply Ability: 10g |
| Release date: 2024/11/07 |
Product Introduction
Bioactivity
| 名稱 | ANA-12 |
| 描述 | ANA-12 is a potent and selective TrkB antagonist that exhibits anxiolytic and antidepressant activity in mice. |
| 細(xì)胞實(shí)驗(yàn) | Modulation of neurite outgrowth by molecules is assessed in nnr5 PC12–TrkB, –TrkA, and –TrkC cells after addition of BDNF (1 nM), NGF (2 nM), and NT-3 (10 nM), respectively. The number of cells bearing neurites longer than 2 cells in diameter in each counting field is microscopically determined (2 fields per well, 3 wells per condition). Counting is performed blind each 24 hours for 3 days. (Only for Reference) |
| 激酶實(shí)驗(yàn) | ANA-12 Binding assay: Maxisorp ELISA 96-well plates are coated with various concentrations of Trk BECD -Fc, 20 mg/ml BSA, or 1 mg/mL IgG-Fc (polyclonal anti-TrkB) in a carbonate buffer (pH 9.6) overnight at 4°C. Plates are saturated with 0.5% BSA in PBS for 2 hours at room temperature and extensively washed in PBS-Tween 0.05%. Bodipy–ANA-12 is then incubated in 0.5% PBS-BSA for 1 hour at room temperature before the addition of BDNF in 0.5% PBS-BSA for another hour. After extensive washes in PBS-Tween 0.05%, the amount of bodipy–ANA-12 bound is quantified by fluorescence at 520 ± 10 nm. Detectability range for extrapolation analysis is assessed by coating ELISA plates with bodipy–ANA-12 and reading fluorescence at 520 ± 10 nm. |
| 體外活性 | In male Sprague-Dawley rats, ANA-12 (3 μg/dose) inhibits the medial nucleus of the solitary tract (mNTS) BDNF's effect on reducing food intake. In male C57BL/6 mice, ANA-12 (0.5 mg/kg, intraperitoneally) demonstrates antidepressant effects against lipopolysaccharide-induced depressive-like behavior. In wild-type male mice, ANA-12 reverses ethanol consumption and induces D3 receptor downregulation; however, it is ineffective in D3R -/- mice. |
| 體內(nèi)活性 | In nnr5 PC12-TrkB cells, ANA-12 inhibits neurite outgrowth induced by brain-derived neurotrophic factor (BDNF) at concentrations as low as 10 nM. It selectively binds directly to the TrkB receptor, suppressing the downstream TrkB pathway without altering the functions of TrkA and TrkC. Furthermore, ANA-12 nullifies the effect of BDNF on increasing the inward current in DRG neurons. |
| 存儲(chǔ)條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | Ethanol : < 1 mg/mL (insoluble or slightly soluble) H2O : < 1 mg/mL (insoluble or slightly soluble) DMSO : 4.07 mg/mL (10 mM), Sonication is recommended. |
| 關(guān)鍵字 | Tropomyosin related kinase receptor | inhibit | Trk Receptor | ANA-12 | Inhibitor |
| 相關(guān)產(chǎn)品 | LM22A-4 | Larotrectinib sulfate | N-Acetyl-5-hydroxytryptamine | Selitrectinib | Sitravatinib | SP600125 | Amitriptyline hydrochloride | 7,8-Dihydroxyflavone | Diosmetin | GW 441756 | CG 428 | ALE-0540 |
| 相關(guān)庫(kù) | 表型篩選靶點(diǎn)鑒定庫(kù) | 經(jīng)典已知活性庫(kù) | 膜蛋白靶向化合物庫(kù) | 酪氨酸激酶分子庫(kù) | 激酶抑制劑庫(kù) | 抑制劑庫(kù) | NO PAINS 化合物庫(kù) | 已知活性化合物庫(kù) |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
You may like
Recommended supplier
| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $2500.00/100mg |
VIP1Y
|
TargetMol Chemicals Inc.
|
2024-10-28 | |
| $20.00/1kg |
VIP1Y
|
Shaanxi Franta Biotechnology Co., Ltd
|
2024-09-23 | |
| $59.00/5g |
VIP4Y
|
Shanghai UCHEM Inc.
|
2023-12-15 | |
| $1.00/1g |
VIP5Y
|
Career Henan Chemical Co
|
2019-12-23 |
United States- Since: 2011-01-07
- Address: 36?Washington?Street, Wellesley?Hills
INQUIRY