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    Postion:Product Catalog >Dyes and Pigments>dye>Acid dye> Sulforhodamine B
    	Sulforhodamine B
    • 	Sulforhodamine B
    • 	Sulforhodamine B

    Sulforhodamine B NEW

    Price $6 $5 $1
    Package 1KG 25KG 100KG
    Min. Order: 1KG
    Supply Ability: g-kg-tons, free sample is available
    Update Time: 2024-03-29

    Product Details

    Product Name: Sulforhodamine B CAS No.: 3520-42-1
    Min. Order: 1KG Purity: 99%
    Supply Ability: g-kg-tons, free sample is available Release date: 2024/03/29
    Lead time: In stock Packaging: bottle/drum/bucket/IBC, as request.
    Delivery: By sea, by air, by express Origin: Manufacturer
    Name: Sun

    1. Product information

    Names

    Namelissamine rhodamine
    SynonymMore Synonyms

     Acid Red 52 Biological Activity

    DescriptionSulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.
    Related Catalog
    Signaling Pathways >> Others >> Others
    Dye Reagents
    Research Areas >> Others
    In VitroSulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1].
    Cell AssayGently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature[1].
    References

    [1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.

     Chemical & Physical Properties

    Molecular FormulaC27H29N2NaO7S2
    Molecular Weight580.65
    PSA150.78000
    LogP7.81310
    Water Solubilitymethanol: 1 mg/mL

     MSDS

    Acid Red 52 MSDS(Chinese)

     Toxicological Information

    CHEMICAL IDENTIFICATION

    • RTECS NUMBER :

    • BP6750000

    • CHEMICAL NAME :

    • Ammonium, (6-(diethylamino)-9-(2,4-disulfophenyl)-3H-xanthen-3- ylidene)diethyl-, hydroxide, inner salt, sodium salt

    • CAS REGISTRY NUMBER :

    • 3520-42-1

    • LAST UPDATED :

    • 199701

    • DATA ITEMS CITED :

    • 5

    • MOLECULAR FORMULA :

    • C27-H29-N2-O7-S2.Na

    • MOLECULAR WEIGHT :

    • 580.69

    HEALTH HAZARD DATA

    ACUTE TOXICITY DATA

    • TYPE OF TEST :

    • LD50 - Lethal dose, 50 percent kill

    • ROUTE OF EXPOSURE :

    • Oral

    • SPECIES OBSERVED :

    • Rodent - mouse

    • DOSE/DURATION :

    • 10300 mg/kg

    • TOXIC EFFECTS :

    • Details of toxic effects not reported other than lethal dose value

    MUTATION DATA

    • TYPE OF TEST :

    • Cytogenetic analysis

    • TEST SYSTEM :

    • Rodent - hamster Fibroblast

    • DOSE/DURATION :

    • 13100 mg/L

    • REFERENCE :

    • FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House, Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year: 22,623,1984 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M2208 No. of Facilities: 114 (estimated) No. of Industries: 5 No. of Occupations: 7 No. of Employees: 901 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M2208 No. of Facilities: 2248 (estimated) No. of Industries: 47 No. of Occupations: 51 No. of Employees: 75084 (estimated) No. of Female Employees: 12598 (estimated)

     Safety Information

    SymbolArticle illustration
    GHS07
    Signal WordWarning
    Hazard StatementsH315-H319-H335
    Precautionary StatementsP261-P305 + P351 + P338
    Personal Protective EquipmentEyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
    Hazard CodesXi
    Risk Phrases36/37/38
    Safety Phrases22-24/25
    RIDADRNONH for all modes of transport
    WGK Germany2
    RTECSBP6750000
    HS Code3204120000

     Precursor & DownStream

    Precursor  0



    DownStream  1



    • Article illustration CAS#:62796-29-6
      lissamine rhoda...

     Customs

    HS Code2921499090
    Summary2921499090 other aromatic monoamines and their derivatives; salts thereof VAT:17.0% Tax rebate rate:9.0% Supervision conditions:none MFN tariff:6.5% General tariff:30.0%

     Articles180

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     Synonyms

    4'-Butoxy-3-piperidinopropiophenone hydrochloride
    Tanaclone
    EINECS 222-529-8
    dyclonine hydrochloride
    SULFORHODAMINE B
    MFCD00010180
    C.I. 45100
    Xanthylium, 3,6-bis(diethylamino)-9-(2,4-disulfophenyl)-, inner salt, sodium salt (1:1)
    dye acid red 52
    Sodium 4-[6-(diethylamino)-3-(diethyliminio)-3H-xanthen-9-yl]benzene-1,3-disulfonate
    Sulforhodamine-B
    Dyclone
    N,N,N',N'-tetraethylsulforhodamine
    rhodamine 200 S sodium salt
    Dyclocaine hydrochloride
    Dyclonine HCL
    Acid Red 52,Sulforhodamine B monosodium salt
    Dyclothane
    Acid Red 52
    C.I.Acid Red 52
    Sulforhodamine B sodium salt
    Sulforhodamine B (sodium salt)


    2. Packaging

    For powders: normal is 25kgs/Drum or bag, or larger/smaller package as request.

    For liquids: normal 25kgs/drum, 180-300kgs/bucket, or IBC, determined by the nature of the product. 

                         Or smaller package 1kg/bottle, 10kgs/bottle as request. 


    Article illustrationArticle illustration


    3. Shipping

    Article illustration


    4. Contact information

    For more details, pls contact us freely.

    Email address : Sun@fdachem.com

    Mob: 86 13526505137

    WhatsApp/Skype/Wechat/LINE: 86 13526505137





    Company Profile Introduction

    Henan Fengda Chemical Co., Ltd. is located in the High-tech Development Zone of Henan Province. Specializing in the production and sales of various fine chemical products required for industrial production, including chemical raw materials, organic raw materials, petrochemicals, chemical reagents, solvents, catalysts, and additives, etc.

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    • Address: Room 01, 2288 E05, Building 14, East Henan University, Science and Technology Park, 279 Xisanhuan Ro
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