PNGASE F
- CAS No.
- 83534-39-8
- Chemical Name:
- PNGASE F
- Synonyms
- PNGASE A;PNGASE F;EC 3.5.1.52;N-GLYCANASE;GLYCOPEPTIDASE;N-GLYCANASE(R);PNGase F(Fast);N-GLYCOSIDASE A;N-GLYCOSIDASE F;GLYCOPEPTIDASE A
- CBNumber:
- CB2114680
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MFCD00131222
- MOL File:
- Mol file
- MSDS File:
- SDS
SAFETY
Risk and Safety Statements
Symbol(GHS) | GHS08 |
---|---|
Signal word | Danger |
Hazard statements | H334 |
Precautionary statements | P261-P284-P501 |
Hazard Codes | Xi,N,Xn |
Risk Statements | 37/38-41-50/53-43-20/21/22 |
Safety Statements | 22-24/25-36/37/39-26-61 |
RIDADR | UN 3316 9 |
WGK Germany | 2 |
PNGASE F price More Price(16)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich | P7367 | PNGase F from Elizabethkingia meningoseptica BioReagent, ≥95% (SDS-PAGE), for proteomics | 83534-39-8 | 50units | $407 | 2024-03-01 | Buy |
Sigma-Aldrich | P7367 | PNGase F from Elizabethkingia meningoseptica BioReagent, ≥95% (SDS-PAGE), for proteomics | 83534-39-8 | 300Units | $1800 | 2024-03-01 | Buy |
Sigma-Aldrich | G5166 | PNGase F from Elizabethkingia miricola buffered aqueous solution | 83534-39-8 | 50units | $330 | 2024-03-01 | Buy |
Sigma-Aldrich | G5166 | PNGase F from Elizabethkingia miricola buffered aqueous solution | 83534-39-8 | 100units | $540 | 2024-03-01 | Buy |
Sigma-Aldrich | G1549 | PNGase F from Elizabethkingia meningoseptica buffered aqueous solution, recombinant, expressed in E. coli | 83534-39-8 | 50units | $272 | 2024-03-01 | Buy |
PNGASE F Chemical Properties,Uses,Production
Uses
Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.
Uses
PNGase F from Elizabethkingia meningoseptica has been used:
- for de-N-glycosylation of Zika20virus E protein
- to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
- to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)
Uses
Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.
General Description
PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.
Biochem/physiol Actions
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
PNGASE F Preparation Products And Raw materials
Raw materials
Preparation Products
PNGASE F Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
GlycoPharm.LLC | +86-27-63498076 | sale@glycolipidpharm.com | CHINA | 158 | 58 |
Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
Career Henan Chemica Co | +86-0371-86658258 +8613203830695 | laboratory@coreychem.com | China | 30241 | 58 |
TargetMol Chemicals Inc. | support@targetmol.com | United States | 38460 | 58 | |
Shanghai Acmec Biochemical Technology Co., Ltd. | +undefined18621343501 | product@acmec-e.com | China | 33338 | 58 |
Aladdin Scientific | +1-+1(833)-552-7181 | sales@aladdinsci.com | United States | 52925 | 58 |
SHANGHAI KEAN TECHNOLOGY CO., LTD. | +8613817748580 | cooperation@kean-chem.com | China | 40066 | 58 |
Wuhan Chemstan Biotechnology Co., Ltd. | 027-65317797 15926423062 | 422450190@qq.com | China | 10351 | 58 |
Wuhan Tangzhi Pharmaceutical Co., Ltd. | 0532-88500810 13658392159 | sale@glycogene.com | China | 837 | 58 |
3B Pharmachem (Wuhan) International Co.,Ltd. | 821-50328103-801 18930552037 | 3bsc@sina.com | China | 15839 | 69 |
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