Fluorescein diacetate NEW
| Price | $42 |
| Package | 500mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-19 |
Product Details
| Product Name: Fluorescein diacetate | CAS No.: 596-09-8 |
| Purity: 99.61% | Supply Ability: 10g |
| Release date: 2024/11/19 |
Product Introduction
Bioactivity
| Name | Fluorescein diacetate |
| Description | Fluorescein diacetate (Di-O-acetylfluorescein) is a cell permeable esterase-substrate for hGSTP1-1. |
| In vitro | Fluorescein diacetate (FDA) serves as a fluorogenic substrate in assessing the efficacy of inhibitors against the enzyme GSTP1-1 in vitro. Notably, Ethacrynic acid (EA) and NBDHEX are effective in inhibiting the enzyme-activated fluorescence of FDA, demonstrating concentration-dependent inhibition with IC50 values of 3.3±0.5 μM and 0.61±0.04 μM, respectively. FDA, an acetylated variant of fluorescein, is activated fluorescently through the esterolytic action of the human Pi-class glutathione S-transferase (hGSTP1), marking it specific among various cytosolic GSTs. This fluorogenic activity allows for the usage of FDA in vital staining. Particularly, when MCF7 cells overexpress hGSTP1, an observable fluorescence activation due to GST inhibitors is noted, an effect not seen in cells overexpressing hGSTA1 or hGSTM1. The enzyme-dependent fluorescence activation is further corroborated by incubating FDA with recombinant hGSTP1-1 and GSH, showing significant fluorescence only in the presence of both, indicating the necessity of hGSTP1-1 activity for such activation. This specificity is underscored by the established linear relation between fluorescence increase rate and hGSTP1-1 concentration, determining the enzyme's specific activity for FDA to be 79±15 nmol/min/mg protein. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 6.88 mg/mL (16.51 mM), Sonication is recommended. |
| Keywords | Inhibitor | Fluorescein diacetate | Fluorescein Diacetate | Fluorescein | inhibit |
| Related Compound Libraries | Bioactive Compound Library | Bioactive Compounds Library Max | Human Metabolite Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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