479413-70-2
中文名稱
AUDA
英文名稱
AUDA
CAS
479413-70-2
分子式
C23H40N2O3
分子量
392.58
MOL 文件
479413-70-2.mol
更新日期
2024/11/05 13:11:13
479413-70-2 結(jié)構(gòu)式
基本信息
中文別名
化合物AUDA12-(3-(金剛烷-1-基)脲基)十二酸
英文別名
AUDA12-(3-adamantan-1-yl-ureido)dodecanoic acid
12-[[(tricyclo[3.3.1.13,7]dec-1-ylamino)carbonyl]amino]-dodecanoic acid
Dodecanoic acid, 12-[[(tricyclo[3.3.1.13,7]dec-1-ylamino)carbonyl]amino]-
物理化學(xué)性質(zhì)
熔點(diǎn)114 °C(Solv: hexane (110-54-3))
沸點(diǎn)592.7±19.0 °C(Predicted)
密度1.09±0.1 g/cm3(Predicted)
儲存條件Sealed in dry,Store in freezer, under -20°C
溶解度DMSO：加熱至60℃時(shí)≥10mg/mL
酸度系數(shù)(pKa)4.78±0.10(Predicted)
形態(tài)粉末
顏色白色至米色
常見問題列表
生物活性
AUDA (compound 43) 是一種有效的 soluble epoxide hydrolase (sEH) 的抑制劑，對小鼠sEH和人sEH的IC50值分別為18 nM和69 nM。AUDA具有抗炎活性，可降低MMP-9、IL-1β、TNF-α 和 TGF-β。AUDA還可下調(diào) Smad3 和 p38 信號通路。靶點(diǎn)
| Target | Value |
|
MMP-9
() | |
|
IL-1β
() | |
|
TNF-α
() | |
|
TGF-β
() | |
|
Smad3
() |
體外研究
AUDA (0.3-10 μg/mL; 48 hours) dose-dependently suppresses the proliferation of rat VSMCs exposed to PDGF.
AUDA (0.3-10 μg/mL; 30 min) dose-dependently upregulats COX-2 expression.
AUDA (10, 50 and 100 μM) augments the migratory ability of HCAECs in a dose-dependent manner.
AUDA significantly increases the adhesion ability of HCAECs.
Cell Proliferation Assay
| Cell Line: | Vascular smooth muscle cell (VSMC) |
| Concentration: | 0.3, 1, 3, 10 μg/mL |
| Incubation Time: | 48 hours |
| Result: | Dose-dependently suppressed the proliferation of rat VSMCs exposed to PDGF. |
Western Blot Analysis
| Cell Line: | VSMC |
| Concentration: | 1, 3, 10, 30 μg/mL |
| Incubation Time: | 30 min |
| Result: | Dose-dependently upregulated COX-2 expression. |
體內(nèi)研究
AUDA (i.p.; 10 mg/kg; 14 days) reduces TNF-α, MMP-9 and IL-1β expression levels.
| Animal Model: | Male (wild-type) C57BL/6 mice (age, 4-6 weeks; weight, 18-20 g) |
| Dosage: | 10 mg/kg |
| Administration: | i.p.; 14 days |
| Result: | Reduced TNF-α, MMP-9 and IL-1β expression levels. |