338404-52-7
338404-52-7 結(jié)構(gòu)式
基本信息
6-(4-氯苯基)咪唑并[2,1-B]噻唑-5-甲醛 O-(3,4-二氯芐基)肟
物理化學(xué)性質(zhì)
安全數(shù)據(jù)
常見問題列表
CAR, PXR
CITCO (1-50 μM; 48?hours) results in a dose-dependent inhibition of viable cell count and proliferation in both T98G and U87MG glioma and BTSCs.
CITCO (2.5, 5?μM; 48?hours) induces cell cycle arrest differentially in different BTSCs in culture, but not in normal astrocytes.
CITCO (2.5-10?μM; 48?hours) induces apoptosis in BTSCs in culture in dose dependently, but not in normal astrocytes.
CITCO (0-25?μM; 48?hours) causes the T98G and U87MG glioma and BTSCs expressing very low levels of CAR protein that increased significantly.
Cell Proliferation Assay
| Cell Line: | T98G, U87MG, DB29 and DB33 human glioma cells, astrocytes |
| Concentration: | 1, 2.5, 5, 10, 25, 50 μM |
| Incubation Time: | 48?hours |
| Result: | Resulted in a dose-dependent inhibition of viable cell count and proliferation. |
Cell Cycle Analysis
| Cell Line: | The T98G, U87MG, DB29 and DB33 glioma cells |
| Concentration: | 2.5, 5?μM |
| Incubation Time: | 48?hours |
| Result: | Induced cell cycle arrest differentially in different BTSCs in culture. |
Apoptosis Analysis
| Cell Line: | The T98G, U87MG, DB29 and DB33 glioma cells |
| Concentration: | 2.5, 5 or 10?μM |
| Incubation Time: | 48?hours |
| Result: | Increased the levels of Annexin V-positive apoptotic cells in dose dependently. |
Western Blot Analysis
| Cell Line: | T98G, U87MG, DB29 and DB33 glioma cells |
| Concentration: | 0 to 25?μM |
| Incubation Time: | 48?hours |
| Result: | The T98G, U87MG glioma and BTSCs expressed very low levels of CAR protein that increased significantly. |
CITCO (intraperitoneal; on days 22, 24, 26, 30 and 36) with 25?μg results a significant decrease in tumour growth, which further decreases to an undetectable level after treatment with 100?μg CITCO .
| Animal Model: | Six- to eight-week-old male athymic nude mice |
| Dosage: | 25 or 100?μg |
| Administration: | Intraperitoneal; on days 22, 24, 26, 30 and 36 |
| Result: | Decreased tumour growth. |