2447-54-3
基本信息
血根堿
血根堿40%
假白屈菜季銨堿
血根堿(標準品)
血根堿.博落回提取物
血根堿(假白屈菜季銨堿)
SANGUINARINE 血根堿
Sangrovit
SANGUINARIN
SANGUINARINE
PSEUDOCHELERYTHRINE
SANGUINARINE, 40+% BY UV
SANGUINARINE, 98+% BY HPLC
dimethylenedioxybenzphenanthridine
Sanguinarine/Macleaya cordata extract
SANGUINARINE CHLORIDE ISOLATED FROM MACL EAY
物理化學性質(zhì)
常見問題列表
圖1為血根堿化學結(jié)構(gòu)式
臨床研究結(jié)果表明,血根堿對口腔中大多數(shù)細菌有抑菌和殺菌作用,并有干擾細菌粘附于剛形成的牙膜上的作用。含血根堿提取物(SaE)/氯化鋅的牙膏和漱口液的臨床試驗表明,用含質(zhì)量分數(shù)為0.075%SaE、質(zhì)量分數(shù)為2.0%或更多的氯化鋅牙膏和含質(zhì)量分數(shù)為0.03%SaE、質(zhì)量分數(shù)為0.2%氯化鋅漱口液進行試驗,7位試驗者牙菌斑全部減少,牙齦健康改善,其中3位經(jīng)歷6個月后見效。短期試驗的效果還有待進一步證實。
血根堿作用機理還需要進一步探討。氯化鋅和血根堿各自對復(fù)配物活性的貢獻還不清楚,市售含血根堿的牙膏是含有陽離子抗菌劑和陰離子表面活性劑(月桂基硫酸酯鈉鹽) 的配方,在該牙膏中血根堿(陽離子抗菌劑)仍然保持其抗菌活性,其原因還不清楚。
以上信息由Chemicalbook彤彤編輯整理。
Apoptosis
Sanguinarine (SANG)-induced apoptosis is associated with the activation of JNK and NF-κB signal pathways.To determine the effects of Sanguinarine on cell viability, 22B-cFluc cells are stimulated with different concentrations of Sanguinarine for 24 h, and then a CKK-8 assay is performed. The treatment with Sanguinarine decreases the proliferation of 22B cells in a dose-dependent manner. Meanwhile, the cytosolic extracts of 22B-cFluc cells treated with different dose of Sanguinarine are measured to detect cellular caspase-3 activity using Ac-DEVD-pNA, which is a validated caspase-3 substrate. The absorbance at 450 nm increases in a dose-dependent manner, indicating increased caspase-3 activity stimulated by Sanguinarine.
To evaluate the apoptosis induced by Sanguinarine (SANG) in vivo, 22B-cFluc cells are inoculated subcutaneously into one flank of nude mice and xenograft models are allowed to establish. Mice are treated intravenously with 10 mg/kg of Sanguinarine. At 24, 48 and 72 h after treatment, bioluminescent imaging is performed after i.p. injection of mice with 150 mg/kg of D-luciferin substrate. Sanguinarine treatment induces an obvious increase of luminescent signal as early as 48 h after initial treatment. A sustained bioluminescent imaging (BLI) intensity increased is observed throughout the course of experiment. At 72 h after treatment, the tumors are collected and subjected to TUNEL staining for evaluating apoptosis. Compared with the control tumors, the group treated with Sanguinarine exhibits significantly more cell apoptosis, indicated by the increased green signals from the sporadic apoptotic cells.