205391-02-2
中文名稱
205391-02-2
英文名稱
4,5-DIAMINOFLUORESCEIN DIACETATE
CAS
205391-02-2
分子式
C24H18N2O7
分子量
446.41
MOL 文件
205391-02-2.mol
205391-02-2 結(jié)構(gòu)式
基本信息
中文別名
化合物 T189664,5-二氨基熒光素二乙酸酯
英文別名
DAF-2 DADAF-2 DIACETATE
DAF-2 DA, CELL PERMEABLE
EZSolution DAF-2 diacetate
5,6-Diaminofluorescein diacetat
4,5-DIAMINOFLUORESCEIN DIACETATE
5,6-DIAMINOFLUORESCEIN DIACETATE
4,5-DIAMINOFLUORORESCEIN DIACETATE
DAF-2 DA - CAS 205391-02-2 - Calbiochem
2-(3,6-DIACETYLOXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID
物理化學(xué)性質(zhì)
沸點(diǎn)719.0±60.0 °C(Predicted)
密度1.54±0.1 g/cm3(Predicted)
閃點(diǎn)87 °C
儲(chǔ)存條件2-8°C
溶解度二甲基亞砜：<2mg/毫升
酸度系數(shù)(pKa)2.92±0.20(Predicted)
形態(tài)淡黃色溶液
顏色白色
常見問(wèn)題列表
生物活性
DAF-2DA (5,6-Diaminofluorescein diacetat) 是測(cè)量 NO 最廣泛使用的一種熒光探針。體外研究
Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N 2 O 3 , which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).