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1678416-08-4

CAS 1678416-08-4
更新日期 2024/11/01 16:18:34
1678416-08-4 結(jié)構(gòu)式 1678416-08-4 結(jié)構(gòu)式

常見問題列表

生物活性
β-Amyloid (1-40), FAM-labeled 是一個 FAM 熒光標記的 β-Amyloid (1-40) 肽 (λex=?492?nm and λem=?518?nm)。
靶點

λ ex =?492?nm; λ em =?518?nm

體外研究

Apical-to-basolateral exchange across endothelial monolayer of fluorescent Aβ42 (FAM-labeled Human β-Amyloid (1-42) and Fluor ? 488-labeled β-Amyloid (1-42)) or scramble Aβ42 ( FAM-labeled scrambled β-Amyloid (1-42) ) (1-100 μM) is monitored in transendothelial electrical resistance (TEER) during cell monolayer’s formation over time (over 120?min in presence or absence of Aβ24). Human β-Amyloid (1-24) (1 μM) results in H-Aβ42 retention, thus reducing its efflux through the BBB and therefore preventing an efficient mechanism of Aβ42 clearance.The in vitro BBB model is used to investigate the passage of FAM-labeled or 488-conjugated H-Aβ42 across the endothelial cell monolayer.?The addition of fluorescently labeled H-Aβ42 (FAM-labeled Human β-Amyloid (1-42) or Fluor ? 488-labeled β-Amyloid (1-42)) to the apical side of cell inserts results in effective BBB crossing, FAM-labeled scrambled β-Amyloid (1-42) is not efficiently transcytosed.

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