152918-18-8
152918-18-8 結(jié)構(gòu)式
基本信息
N6-(3-碘芐基)腺苷-5'-N-甲基糖酰胺
CS-2564
IB-MECA
RPR-113090
Selepressin
PICLIDENOSON
CF-101
Piclidenoson (IB-MECA)
IBMECA
(PICLIDENOSON
CF-101
IB-MECA SELECTIVE A3 ADENOSIN
N6-(3-IODOBENZYL)ADO-5'N-METHYLURONAMIDE
物理化學(xué)性質(zhì)
應(yīng)用領(lǐng)域
常見問題列表
A3AR
Piclidenoson is able to inhibit Forskolin (HY-15371)-stimulated cAMP levels with EC
50
s of 0.82 μM and 1.2 μM in OVCAR-3 cells and Caov-4 cells, respectively.
Piclidenoson (0.0001-100 μM; 48 hours) significantly reduces cell viability in a dose-dependent manner in human ovarian cancer cell lines, with IC
50
s of 32.14 μM and 45.37 μM for OVCAR-3 and Caov-4 cells, respectively.
Piclidenoson (0.001-100 μM; 48 hours) induces apoptosis in ovarian cancer cell line through the caspase pathway.
Piclidenoson induces apoptosis via the mitochondrial signaling pathway.
Cell Proliferation Assay
| Cell Line: | OVCAR-3 cells, Caov-4 cells |
| Concentration: | 0.0001-100 μM |
| Incubation Time: | 48 hours |
| Result: | Resulted in a dose-dependent reduction in the cell viability. |
Apoptosis Analysis
| Cell Line: | OVCAR-3 cells, Caov-4 cells |
| Concentration: | 0.1 μM, 1 μM, 10 μM, 50 μM, 100 μM |
| Incubation Time: | 48 hours |
| Result: | Significant increased in the percentage of apoptosis in a concentration-dependent manner. |
Western Blot Analysis
| Cell Line: | OVCAR-3 cells, Caov-4 cells |
| Concentration: | 1 μM, 10 μM, 100 μM |
| Incubation Time: | 48 hours |
| Result: | Decreased the expression of Bcl-2 was noticeably and increased the expression of Bax protein. |
Piclidenoson (105 μg/kg; i.p.) enhances survival of γ-irradiated mice.
| Animal Model: | B10CBAF1 male mice aged 3 months (average 30 g) |
| Dosage: | 105 μg/kg |
| Administration: | Intraperitoneal injection, 0.5 h after irradiation |
| Result: | Resulted in statistically significant increases of the mean survival time in comparison with the control irradiated mice. |