1202580-59-3
1202580-59-3 結構式
基本信息
CAY10618
CAY10618 Exclusive
N-(2-phenylphenyl)-8-(4-pyridin-3-yltriazol-1-yl)octanamide
1H-1,2,3-Triazole-1-octanaMide, N-[1,1'-biphenyl]-2-yl-4-(3-pyridinyl)-
物理化學性質
常見問題列表
Nampt;
Autophagy
GPP78 (Compound 8; 10 nM; 24-40 hours; SH-SY5Y cells) treatment with cells, punctate staining of LC3-II and the formation of autophagolysosomes are observable. LC3-II is membrane-bound and is present in autophagosomes.
GPP78 (Compound 8) inhibits the growth of most cell lines tested, with nanomolar potency (GI
50
) in cell lines derived from leukemia, lung, CNS, colon, melanoma, ovarian, renal, and prostate cancers. GPP78 appears truly cytotoxic in melanoma cell lines, while in the others it is mainly cytostatic.
Western Blot Analysis
| Cell Line: | SH-SY5Y cells |
| Concentration: | 10 nM |
| Incubation Time: | 24 hours, 40 hours |
| Result: | Punctate staining of LC3-II and the formation of autophagolysosomes were observable. |
GPP78 (10 mg/kg; intraperitoneal injection; daily; 1 hour or 6 hours after SCI; for 19 days; male adult CD1 mice) treatment reduces the severity of spinal cord trauma in SCI mice.
| Animal Model: | Male adult CD1 mice (25-30 g) with spinal cord injury (SCI) |
| Dosage: | 10 mg/kg |
| Administration: | Intraperitoneal injection; daily; 1 hour or 6 hours after SCI; for 19 days |
| Result: | Significantly reduced the demyelination associated with SCI. And significantly ameliorated the functional deficits induced by SCI. |