108340-60-9
中文名稱
靈芝酸D
英文名稱
Ganoderic acid D
CAS
108340-60-9
分子式
C30H42O7
分子量
514.65
MOL 文件
108340-60-9.mol
更新日期
2025/01/14 08:54:22
108340-60-9 結(jié)構(gòu)式
基本信息
中文別名
靈芝酸D靈芝酸D標(biāo)準(zhǔn)品
靈芝酸D對(duì)照品,
英文別名
(7beta)-7-Hydroxy-3,11,15,23-tetraoxolanost-8-en-26-oic acidLanost-8-en-26-oic acid, 7-hydroxy-3,11,15,23-tetraoxo-, (7β)-
所屬類別
有機(jī)原料：羧酸類化合物及衍生物物理化學(xué)性質(zhì)
外觀性狀白色粉末，可溶于甲醇、乙醇、DMSO等有機(jī)溶劑，來(lái)源于多孔菌科靈芝Ganodermalucidum（Leyss.exFr.）Karst．的干燥子實(shí)體。
沸點(diǎn)688.3±55.0 °C(Predicted)
密度1.22±0.1 g/cm3(Predicted)
儲(chǔ)存條件Store at -20°C, protect from light
溶解度Soluble in methanol;
酸度系數(shù)(pKa)4.77±0.23(Predicted)
形態(tài)粉末
顏色白色
水溶解性不溶于水
常見(jiàn)問(wèn)題列表
生物活性
Ganoderic acid D 是一種高度氧化的四環(huán)三萜類化合物 (tetracyclic triterpenoid)，是靈芝 (Ganoderma lucidum) 的主要活性成分。Ganoderic acid D 上調(diào) SIRT3 的蛋白質(zhì)表達(dá)并通過(guò) SIRT3 誘導(dǎo)脫乙酰化的親環(huán)蛋白 D (CypD)。Ganoderic acid D 抑制結(jié)腸癌細(xì)胞的能量重編程，包括結(jié)腸癌細(xì)胞中的葡萄糖攝取，乳酸產(chǎn)生，丙酮酸和乙酰輔酶的產(chǎn)生。Ganoderic acid D 誘導(dǎo) HeLa 人宮頸癌細(xì)胞凋亡 (apoptosis)。靶點(diǎn)
|
SIRT3
|
體外研究
Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC
50
of 17.3 mM.
Ganoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells.
Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3.
Cell Viability Assay
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 1, 5, 10, 20, 50 μM |
| Incubation Time: | 24, 48, 72 hours |
| Result: | Reduced the cell survival rate in a dose- and time-dependent manner and had an IC 50 value of 17.3 μM for 48 hours treatment. |
Cell Cycle Analysis
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 10, 50 μM |
| Incubation Time: | 24, 48 hours |
| Result: |
Induced G2/M phase arrest.
Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM. |
Apoptosis Analysis
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 10, 50 μM |
| Incubation Time: | 48 hours |
| Result: | Induced a morphological change typical of apoptosis in HeLa cells. |
Western Blot Analysis
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 10 μM |
| Incubation Time: | 48 hours |
| Result: | Up-regulated 14-3-3E and PRDX3. |