100874-08-6
中文名稱
SB 4
英文名稱
SB 4
CAS
100874-08-6
分子式
C14H10BrNOS
分子量
320.2
MOL 文件
100874-08-6.mol
更新日期
2024/11/09 19:15:10
100874-08-6 結(jié)構(gòu)式
基本信息
中文別名
化合物SB 42-((4-溴芐基)硫代)苯并[D]惡唑
英文別名
SB 4BMP signaling agonist sb4
Benzoxazole, 2-[[(4-bromophenyl)methyl]thio]-
物理化學(xué)性質(zhì)
熔點(diǎn)70-72 °C
沸點(diǎn)444.2±47.0 °C(Predicted)
密度1.57±0.1 g/cm3(Predicted)
儲(chǔ)存條件2-8°C
儲(chǔ)存條件Sealed in dry,2-8°C
溶解度溶于二甲基亞砜
酸度系數(shù)(pKa)0.92±0.10(Predicted)
形態(tài)Solid
顏色White to light yellow
常見問題列表
生物活性
SB 4 (Eticovo) 是一種 bone morphogenetic protein 4 (BMP4) signaling 的有效的選擇性激動(dòng)劑，其EC50值為74 nM。SB 4 (Eticovo) 可增強(qiáng)經(jīng)典的BMP信號(hào)傳導(dǎo)并激活 SMAD-1/5/9 磷酸化。靶點(diǎn)
| Target | Value |
|
BMP4
(Cell-free assay) | 74 nM(EC50) |
體外研究
BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions. BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4.
Western Blot Analysis
| Cell Line: | Primary mouse kidney epithelial cells (PRECs) |
| Concentration: | 0.05 μM-1 μM |
| Incubation Time: | 24 hours |
| Result: | Incresed p-SMAD-1/5/9 abundance in PRECs. |
Western Blot Analysis
| Cell Line: | BRE-Luc cells |
| Concentration: | 1 μM |
| Incubation Time: | 0min, 5 mins, 15 mins, 30 mins, 45 mins, 60 mins |
| Result: | Acted to stabilize p-SMAD-1/5/9 to enhance the transcriptional response. |