| Identification | Back Directory | [Name]
Aprotinin | [CAS]
9087-70-1 | [Synonyms]
BPTI
fosten
rp-9921
trazinin
zymofren
onquinin
repulson
riker52g
TRASYLOL
antikrein
antilysin
APROTININ
Antilysi.
Aids043659
antilysine
bayera-128
kirrichter
Aids-043659
EC: 3.4.21.9
APROTININ-AGAROSE
APROTININ, BOVINE
Trypsin Inhibitor
Protease inhibitor
Aprotinin, lyophil.
RecoMbinant aprotinin
APROTININ BOVINE LUNG
aprotininfrombovinelung
Aprotinin solution
Kunitz protease inhibitor
TRYPSIN INHIBITOR (BASIC)
pancreatictrypsininhibitor
Anti-Acetylated Lysine:HRP
Aprotinin(Trypsin Inhibitor)
kallikrein-trypsininactivator
TRYPSIN INHIBITOR, BOVINE LUNG
APROTININ, BOVINE, RECOMBINANT
Anti-Acetylated Lysine: Biotin
EZNA KIT ENDO-FREE PLASMID GIGA
basicpancreatictrypsininhibitor
pancreaticbasictrypsininhibitor
PROTEASE INHIBITOR, BOVINE LUNG
trypsininhibitor,pancreaticbasic
TRYPSIN INHIBITOR, PANCREAS TYPE
pancreatictrypsininhibitor(kunitz)
APROTININ-AGAROSE FROM BOVINE LUNG
Bovine pancreatic trypsin inhibitor
trypsin-kallikreininhibitor(kunitz)
APROTININ, BOVINE AGAROSE CONJUGATE
Kunity Trypsin Inhibitor of Aprotinium
Trypsin Inhibitor, pankreatisch basisch
Aprotinin from bovine lung, ca. 140 U/mg
Aprotinin from bovine lung, >=5400 KIU/mg
APROTININ FROM BOVINE LUNGAFFINITY PURIF IED
APROTININ FROM BOVINE LUNG CELL CULTURE TESTED
APROTININ FROM BOVINE LUNG, LYOPH.,~7000 U/MG*
APROTININ FROM BOVINE LUNG, ~8000 U/MG P ROTEIN
Aprotinin froM bovine lung, ca. 140 U/Mg lyophil.
Anti-Acetylated Lysine antibody produced in rabbit
APROTININ, BOVINE LUNG FOR GELFILTRATION CHROMATOG
Aprotinin from bovine lung,Trypsin inhibitor (basic)
Anti-Acetylated Lysine-Biotin antibody produced in rabbit
Rpdfcleppytgpckariiryfynakaglcqtfvyggcrakrnnfksaedcmrtcgga
Monoclonal Anti-Acetylated Lysine antibody produced in mouse
Anti-Acetylated Lysine-Peroxidase antibody produced in rabbit
BPI, BPTI, Basic Protease Inhibitor, Pancreatic Trypsin Inhibitor
H-ARG-PRO-ASP-PHE-CYS-LEU-GLU-PRO-PRO-TYR-THR-GLY-PRO-CYS-LYS-ALA-ARG-ILE-ILE-ARG-TYR-PHE-TYR-ASN-ALA-LYS-ALA-GLY-LEU-CYS-GLN-THR-PHE-VAL-TYR-GLY-GLY-CYS-ARG-ALA-LYS-ARG-ASN-ASN-PHE-LYS-SER-ALA-GLU-ASP-CYS-MET-ARG-THR-CYS-GLY-GLY-ALA-OH | [EINECS(EC#)]
232-994-9 | [Molecular Formula]
C284H432N84O79S7 | [MDL Number]
MFCD00130541 | [MOL File]
9087-70-1.mol | [Molecular Weight]
6511.44 |
| Chemical Properties | Back Directory | [Appearance]
white powder | [Melting point ]
>234oC (dec.) | [storage temp. ]
2-8°C
| [solubility ]
glycerol: soluble3mg/mL, clear, colorless (equilibration buffer containing 5% glycerol) | [form ]
lyophilized powder
| [color ]
white
| [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Water Solubility ]
Freely soluble in water and in aqueous buffers of low ionic strength. | [Merck ]
13,761 | [Sequence]
H-Pro-Asp-Phe-Cys-Leu-Glu-Pro-Pro-Tyr-Thr-Gly-Pro-Cys-Lys-Ala-Arg-Ile-Ile-Arg-Tyr-Phe-Tyr-Asn-Ala-Lys-Ala-Gly-Leu-Cys-Gln-Thr-Phe-Val-Tyr-Gly-Gly-Cys-Arg-Ala-Lys-Arg-Asn-Asn-Phe-Lys-Ser-Ala-Glu-Asp-Cys-Met-Arg-Thr-Cys-Gly-Gly-Ala-OH | [InChIKey]
RWLBZZCCAZDWSE-BNRYNCRGNA-N |
| Hazard Information | Back Directory | [Chemical Properties]
white powder | [Uses]
Aprotinin is largely used as an inhibitor of trypsin. | [Uses]
Proteolytic inhibitor in radioimmunoassays of polypeptide hormones. | [Description]
Aprotinin is a reversible inhibitor of serine proteases such as trypsin (Ki = 0.06 pM), chymotrypsin (Ki = 9.5 nM), and kallikrein (Ki = 0.8 nM).1 It is a much weaker inhibitor of thrombin (Ki = 0.1-0.8 mM) and trypsinogen (Ki = 2 μM).1 Aprotinin also competitively inhibits nNOS and iNOS with Ki values of 50 and 78 μM, respectively.2 Aprotinin is widely used as a protein purification tool to prevent proteases present in tissue samples from degrading the protein of interest. | [Originator]
Trasylol,Bayer | [Manufacturing Process]
1 kg of parotid of cattle, which were freed of fat and flesh are comminuted in
a meat chopper and twice extracted with 5 L of acetone. The acetone was
removed as far as possible by sucking off and the moist material is digested
for 2.5 hours in 1.2 L of 1 N acetic acid and 2.8 L of 96% ethanol at 50°C.
The filtrate containts about 230.000 inactivator units.
The alcoholic solution is concentrated to 1.2 L by evaporating in vacuo and
shaken with an equal amount of ether. Dark coloring matters and residues of
fat are thus dissolved. The aqueous phase is now mixed with acetone until a
precipitate occurs and the dissolved in dilute acetic acid. The solution is
brought to a weakly alkaline pH by addition of ammonia. It is then centrifuged
and the inactivator is precipitated with five times the amount of ethanol. The
yield amounts to about 180.000 kallikrein inactivator units in 0.4-0.5 g of
substance. | [Brand name]
Trasylol (Bayer). | [Therapeutic Function]
Proteinase inhibitor | [General Description]
Trypsin inhibitor, pancreas type from bovine lung. It is known as Pancreatic trypsin inhibitor (BPTI). Aprotinin, also known as pancreatic trypsin inhibitor and trypsin-kallikrein inhibitor, is found to be expressed in lungs, spleen, liver, and pancreas. It is also found to be present in the free form in calf serum. | [Biochem/physiol Actions]
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges. | [Safety Profile]
Experimental reproductive effects. When heated to decomposition it emits toxic fumes of NOx and SOx. | [storage]
Store at 2-8°C | [References]
1. henry da, carless pa, moxey aj, et al. anti-fibrinolytic use for minimising perioperative allogeneic blood transfusion. cochrane database syst rev 2007; 4: cd001886.2. levi m, cromheecke me, de jonge e, et al. pharmacological strategies to decrease excessive blood loss in cardiac surgery: a meta-analysis of clinically relevant endpoints. lancet 1999; 354: 1940-7.3. sedrakyan a, treasure t, elefteriades ja. effect of aprotinin on clinical outcomes in coronary artery bypass graft surgery: a systematic review and meta-analysis of randomized |
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