| Identification | Back Directory | [Name]
Bovine albumin | [CAS]
9048-46-8 | [Synonyms]
nhsa
BSAV
BSA-C
AC-BSA
ALBUMIN, PIG
albumenpowder
SERUM ALBUMIN
ACETYLATED BSA
BSA ACETYLATED
Albumin equine
Bovine albumin
Albuminexbovine
BSA, METHYLATED
BSA-C ACETYLATED
AlbuMin Bovine V
albumin from goat
Rat albumin, RSA
ALBUMIN ACETYLATED
Albumins,bloodserum
compound amino acid
albumin from donkey
Bovine serum albumin
albumin from hamster
Albumin, from Bovine
Albumin BovineⅤ(BSA)
Albumin from chicken
Albumin, Bovine, F-Ⅴ
Albumin powder (egg)
AlbuminBovine(Ph7.0)
Albumin,Bovine(Ph5.2)
ALBUMIN, 35% SOLUTION
Almond protein powder
Albumine bovine cryst.
Cohn fraction V powder
albumin from dog serum
Albumin,bovineSL-5grade
Bovinealbumin,SL-5grade
Albumin,Bovine,Fract V
ALBUMIN FROM RAT SERUM*
albumin from mouse serum
albumin from sheep serum
albumin from canine serum
GARDNER COLOUR 10 (GAR10)
GARDNER COLOUR 12 (GAR12)
BSA=Bovineserumalbumin/CH
ALBUMIN FROM BOVINE SERUM
ALBUMIN RABBIT FRACTION V
ALBUMIN BABOON FRACTION V
ALBUMIN, PIG GLOBULIN-FREE
ALBUMIN BOVINE 5% SOLUTION
ALBUMIN FROM RABBIT SERUM*
AlbuminBovineSerum0.5Mg/Ml
Albumin,Bovine,Crystalline
albumin from porcine serum
GLYCINE BIOTECHNOLOGY GRADE
KIT MAG-BIND FFPE TOTAL RNA
Bovine Serum Albumin BSA-V
ALBUMIN BOVINE 22% SOLUTION
ALBUMIN, BOVINE, METHYLATED
ALBUMIN, BOVINE, ACETYLATED
ALBUMIN, MOUSE GLOBULIN FREE
ALBUMIN, BOVINE 35% SOLUTION
BovinealbuminFr.V,Enzymefree
AlbuminDogCohnFractionV,>95%
AlbuminRatCohnFractionV,>95%
BSA for protein quantitation
BOVINE PLASMA ALBUMIN (BPLA)
Albumin bovine Nuclease-free
Albumin bovine, Low Endotoxin
albumin from guinea pig serum
Albumin from rhesus monkey
Bovine SeruM AlbuMin solution
BOVINE ALBUMINE ( FRACTION V)
AlbuminBovineCrystalline,>98%
ALBUMIN PIG FRACTION V POWDER
ALBUMIN DOG FRACTION V POWDER
ALBUMIN, GOAT FATTY ACID FREE
ALBUMIN RAT FRACTION V POWDER
ALBUMIN, BOVINE, GLOBULIN FREE
METHYL ESTER OF BOVINE ALBUMIN
ALBUMIN MOUSE FATTY ACID FREE
ALBUMIN, RABBIT, GLOBULIN FREE
ALBUMIN GOAT FRACTION V POWDER
AlbuminHorseCohnFractionV,>95%
AlbuminHumanCohnFractionV,>95%
AlbuminMouseCohnFractionV,>95%
Albumin, Bovine, EIA/RIA Grade
Albumin BovineⅤ, No fatty acid
ALBUMIN BOVINE CRYST. LYOPHIL.
ALBUMIN, BOVINE, PROTEASE FREE
bovine,crystalline,lyophilized
Albumin from bovine serum (BSA)
Albumin, from bovine Fatty free
Albumin Bovine, Whole component
BovineAlbumineFractionV(Powder)
AlbuminBovineCohnFractionV,>98%
AlbuminRabbitCohnFractionV,>95%
ALBUMIN, PIG FROM PORCINE SERUM
ALBUMIN HORSE FRACTION V POWDER
ALBUMIN MOUSE FRACTION V POWDER
AlbuminChickenCohnFractionV,>95%
AlbuminPorcineCohnFractionV,>95%
ALBUMIN, BOVINE, 1X CRYSTALLIZED
Albumin (calf serum acetylated)
ALBUMIN BOVINE FRACTION V POWDER
BOVINE SERUM ALBUMIN, ACETYLATED
AlbuMin,bovine standard solution
Albumin from bovine serum (BSA V)
Albumin bovine Fraction V, pH 5.2
Albumin bovine Fraction V, pH 7.0
ALBUMIN BOVINE CELL CULTURE GRADE
ALBUMIN, BOVINE SERUM, METHYLATED
ALBUMIN, SHEEP, FRACTION V POWDER
ALBUMIN, DONKEY FRACTION V POWDER
ALBUMIN FROM DOG SERUM, FRACTION V
AlbuminGuineaPigCohnFractionV,>95%
ALBUMIN, HAMSTER FRACTION V POWDER
ALBUMIN, BOVINE SERUM-C ACETYLATED
albumin solution from bovine serum
AlbuMin, froM bovine Protease free
Albumin from Bovine Serum solution
ALBUMIN, BOVINE, MASS SPEC STANDARD
AlbuminChickenEgg5XCrystalline,>98%
AlbuminChickenEgg2XCrystalline,>94%
ALBUMIN, GUINEA PIG FATTY ACID FREE
BOVINE SERUM ALBUMIN, NON-ACETYLATED
ALBUMIN, BOVINE, CELL CULTURE TESTED
Bovine seruMalbuMin(fifth coMponent)
CAESIUM CHLORIDE, MOL. BIOLOGY GRADE
Albumin,from bovine pH 7.0 Fatty free
ALBUMIN, BOVINE, ESSENTIALLY IGG FREE
AlbuminHumanStandardForElectrohoretic
ALBUMIN, GUINEA PIG FRACTION V POWDER
ALBUMIN FROM SHEEP SERUM, FRACTION V*
ALBUMIN FROM BOVINE SERUM, FRACTION V*
ALBUMIN FROM RABBIT SERUM, FRACTION V*
ALBUMIN, RAT ESSENTIALLY GLOBULIN FREE
ALBUMIN FROM BOVINE SERUM, LYOPHILIZED
Albumin fraction V (from bovine blood)
Albumin bovine Fraction V, Enzyme-free
Albumin from mouse serum,Mouse albumin
Bovinealbumin,cellculturegrade,lyophil.
Bovine albumin, BSA, Cohn FractionV
Albumin from rat serum,RSA, Rat albumin
ALBUMIN BOVINE FRACTION VRECEPTOR GRADE
ALBUMIN, BOVINE FRACTION V LOWENDOTOXIN
Albumin, Acetylated from bovine serum
ALBUMIN, BOVINE, IGG FREE, 30% SOLUTION
ALBUMIN FROM RAT SERUM, FATTY ACID FREE
ALBUMIN, BOVINE FRACTION V PROTEASEFREE
ALBUMIN, BOVINE SERUM 10% SOLUTION CELL
ALBUMIN, RAT ESSENTIALLY FATTY ACIDFREE
ALBUMIN BOVINE FRACTION V POWDER 96-99%
ALBUMIN BOVINE 30% SOLUTION ULTRA HIGH
ALBUMIN BOVINE FRACTION V 35% SOLUTION&
ALBUMIN RHESUS MONKEY FRACTION V POWDER
ALBUMIN BOVINEMICROBIOLOGICAL GRADE PURE
Albumin crystallized (from bovine blood)
Albumin bovine Cohn Fraction V, pH 7.0
MULLER-KAUFFMANN TETRATH.-NOV.BROTH(ISO)
AlbuMin, froM bovine pH 7.0 Low endotoxin
ALBUMINBOVINE,FRACTIONV,LYOPHILIZEDPOWDER
Albumin low endotoxin (from bovine blood)
albumin from bovine serum,protein standard
Albumin bovine Fraction V, Fatty Acid Free
Bovine Serum Albumin, Standard Grade, Gold
ALBUMIN, BOVINE FATTY ACID CONTENT<0.005 %
ALBUMIN, SHEEP ESSENTIALLY FATTY ACIDFRE E
ALBUMIN BOVINE FOR SDS GELELECTROPHORESI S
ALBUMIN BOVINE ESSENTIALLY FATTY ACIDFRE E
ALBUMIN FROM GUINEA-PIG SERUM, FRACTION V*
ALBUMIN BOVINE RIA GRADE FRACTIONV POWDE R
ALBUMIN, RABBIT ESSENTIALLY FATTY ACIDFR EE
ALBUMIN SOLUTION FROM BOVINE SERUM, 300 G/L
ALBUMIN, PIG, FATTY ACID FREE, GLOBULIN FREE
ALBUMIN, RAT, FATTY ACID FREE, GLOBULIN FREE
Albumin, Acetylated from bovine serum,Ac-BSA
ALBUMIN BOVINE COHN FR. V pH7STANDARD GRADE
Albumin from bovine serum,BSA, Bovine albumin
ALBUMIN, BOVINE PLASMA, FOR MOLECULAR BIOLOGY
Albumine bovine, fract. V, very low endotoxin
ALBUMIN, BOVINE 30% SOLUTIONWITH STABILI ZERS
ALBUMIN FROM BOVINE SERUM, LOW IN FATTY ACIDS
BovineAlbumineFractionV(Powder)(AlbuminBovin)
Albumin,bovineFractionV,enzymefreelyophilized
ALBUMIN, GUINEA PIG, ESSENTIALLYGLOBULIN FREE
BOVINE SERUM ALBUMIN MOLECULAR BIOLOGYRE AGENT
ALBUMIN FROM BOVINE SERUM, LOW IN VITAMIN B12*
ALBUMIN, BOVINE FRACTION V POWDEREMBRYO TESTED
ALBUMIN BOVINE 25% SOLUTION IN TYRODE'S BUFFER
Albumin bovine, 30 % solution polymer enhanced
Albumin bovine, Fraction V, Very Low Endotoxin
ALBUMIN BOVINE FRACTION VpH 7.0 STANDARD GRADE
ALBUMIN BOVINE fraction V powder pH 5.0 to 5.2
ALBUMIN BOVINE fraction V powder pH 6.0 to 7.0
Albumin, from bovine serum, fraction V (pH 7.0)
Albumin bovine Fraction V, Enzyme-free lyophil.
ALBUMIN, BOVINE FRACTION V POWDERLOW END OTOXIN
ALBUMIN BOVINE SERUM APPROXMOL WT 66000- 132000
ALBUMIN, BOVINE, FATTY ACID FREE,GLOBULI N FREE
ALBUMIN BOVINE (FRACTION V) FOR ELECTROPHORESIS
ALBUMIN SOLUTION FROM BOVINE SERUM, 5% IN WATER*
ALBUMIN, BOVINE FRACTION V, 30% AQUEOUS SOLUTION
ALBUMIN FROM BOVINE SERUM, CRYSTALLIZED, LYOPH.
Albumin bovine, modified Cohn Fraction V, pH 5.2
Albumin bovine, modified Cohn Fraction V, pH 7.0
Albumin bovine Fraction V, pH 7.0, Low Endotoxin | [EINECS(EC#)]
232-936-2 | [Molecular Formula]
N/A | [MDL Number]
MFCD01114300 |
| Chemical Properties | Back Directory | [Appearance]
Crystalline powder | [RTECS ]
MT6446000 | [storage temp. ]
2-8°C
| [solubility ]
PBS: >40 mg/mL
| [form ]
lyophilized powder
| [color ]
clear clear, colorless | [PH]
6.5-7.5 (1% in 0.15 M sodium chloride) | [Stability:]
Stable. Incompatible with strong oxidizing agents and strong acids. Refrigerate. | [Water Solubility ]
Soluble in water. | [Merck ]
13,8542 | [EPA Substance Registry System]
Albumins, blood serum(9048-46-8) |
| Hazard Information | Back Directory | [Chemical Properties]
Crystalline powder | [Uses]
Suitable for cell culture media supplement. | [Description]
Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids, BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
This BSA product is specifically evaluated and tested for the following properties:
Very low protease content / protease-free
Very low fatty acid content / fatty acid-free
Protease-free BSA is especially useful for applications like:
Enzyme assays
Protein-based assays
Protease-sensitive techniques that include:
a. Enzyme immunoassay (EIA)
b. Nucleic acid hybridization
c. Radioimmunoassay (RIA) | [Application]
Bovine Serum Albumin has been used:
as a constituent of medium used for the differentiation of human pluripotent stem cells
in enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA
to permeabilize fetal cortical cells
This Sigma A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)). | [Definition]
The word bovine means “cow,” and bovine serum albumin (BSA) is a protein that comes from cows. In particular, BSA is a type of protein called albumin, which is found in large amounts in the blood of cows. Blood is a mixture of red blood cells, which is why blood looks red, and many different types of proteins that are dissolved in water. If the cells in blood were removed, you would be left with a clear liquid, called serum. Thus, BSA is the albumin protein that is found in the blood of cows. In the blood, the job of albumin is to carry other proteins from place to place. | [General Description]
Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:
- Protein quantitation (as a standard)
- Immunochemistry (as a blocking agent)
- Cell culture (as a nutrient)
This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
- Enzyme assays
- Protein-based assays
- Protease-sensitive techniques that include:
a) Enzyme immunoassay (EIA)
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA) | [Biological Activity]
Bovine Serum Albumin is a BSA (bovine serum albumin) is a widely used blocking reagent for use in immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.? | [Biochem/physiol Actions]
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. | [storage]
Store at +4°C | [Purification Methods]
Albumin is purified by dissolving it in conductivity water and passage at 2-4o through two ion-exchange columns, each containing a 2:1 mixture of anionic and cationic resins (Amberlite IR-120, H-form, Amberlite IRA-400, OH -form). This treatment removes ions and lipid impurities. Care is taken to exclude CO2, and the solution is stored at -15o. [M.ller et al. Trans Faraday Soc 57 312 1961.] More complete lipid removal is achieved by lyophilising the de-ionised solution, covering the dried albumin (human serum) with a mixture of 5% glacial acetic acid (v/v) in iso-octane (previously dried with Na2SO4) and allowing it to stand at 0o (without agitation) for upwards of 6hours before decanting and discarding the extraction mixture, washing with iso-octane, re-extracting, and finally washing twice with iso-octane. The purified albumin is dried under vacuum for several hours, then dialyzed against water for 12-24hours at room temperature, lyophilised, and stored at -10oC [Goodman Science 125 1296 1957]. It has been recrystallised in high (35%) and in low (22%) EtOH solutions from Cohn's Fraction V. The high EtOH recrystallisation is as follows: To 1kg of Fraction V albumin paste at -5o is added 300mL of 0.4 M pH (pH 5.5) acetate buffer in 35% EtOH pre-cooled to -10o and 430 mL of 0.1 M NaOAc in 25% EtOH also at -10o. Best results are obtained by adding all of the buffer and about half of the NaOAc and stirring slowly for 1hour. The rest of the NaOAc is added when all the lumps have disintegrated. The mixture is set aside at -5o for several days to crystallise. 35% EtOH (1 L) is then added to dilute the crystalline suspension and lower the ionic strength prior to centrifugation at -5o (yield 80%). The crystals are further dissolved in 1.5 volumes of 15% EtOH/0.02M NaCl at -5o and clarified by filtration through washed, calcined diatomaceous earth. This solution may be recrystallised by re-adjusting to the conditions in the first crystallisation, or it may be recrystallised at 22% EtOH with the aid of a very small amount of decanol (enough to give a final concentration of 0.02%). Note that crystallisation from lower EtOH concentration gave better purification (i.e. by removing globulins and carbohydrates) and producing a more stable product. The low EtOH recrystallisation is as follows: To 1kg of Fraction V at -10o to -15o is added 500mL of 15% EtOH at -5o, stirred slowly until a uniform suspension is formed. To the 15% EtOH (500mL) is added sufficient 0.2M NaHCO3 solution (125-150mL) at 0o to bring the pH (1:10 dilution) to 5.3. Some temperature rise occurs, and care must be taken to keep the temperature < -5o. If the albumin is incompletely dissolved a small amount of H2O is added (100mL at a time at 0o, allowing 15minutes between additions). Undissolved albumin can be easily distinguished from small amounts of undissolved globulins, or as the last albumin dissolves, the appearance of the solution changes from milky white to hazy grey-green in colour. Keep the solution at -5o for 12hours and filter by suspending in 15g of washed fine calcined diatomaceous earth, and filtering using a Büchner funnel precoated with coarser diatomaceous earth. The filtrate may require two or more similar filtrations to give a clear solution. To crystallise the filtrate, add through a capillary pipette, and with careful stirring, 1/100volume of a solution containing10% decanol and 60% EtOH (at -10o), and seed with the needle-type albumin crystals. After 2-3days, crystallisation is complete. The crystals are centrifuged off. These are suspended with gentle mechanical stirring in one-third their weight of 0.005 M NaCl pre-cooled to 0o. With careful stirring, H2O (at 0o) is added slowly in an amount equal to 1.7 times the weight of the crystals. At this stage there is about 7% EtOH, and the temperature cannot be made lower than -2.5o to -1o. Clarify, and collect as above. [Cohn et al. J Am Chem Soc 69 1753 1947.] Human serum albumin has been purified similarly with 25% EtOH and 0.2% decanol. The isoelectric points of bovine and human serum albumins are 5.1 and 4.9, respectively. |
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