| Identification | Back Directory | [Name]
FREUND'S COMPLETE ADJUVANT | [CAS]
9007-81-2 | [Synonyms]
FCA
F5881
abs9270
FREUND'S COMPLETE AD
FREUNDS COMPLETE ADJUVANT
FREUND'S COMPLETE ADJUVANT
ADJUVANT, COMPLETE(FREUND)
FREUND'S ADJUVANT COMPLETE
FREUND'S COMPLETE ADJUVANT
Freund's Adjuvant Complete,FCA
IMJECT FREUND'S COMPLETE ADJUVANT
ADJUVANT, COMPLETE, FREUND, H37 RV
Freunds Complete Adjuvant, FCA/CFA)
FREUND'S COMPLETE ADJUVANT USP/EP/BP
Immunostimulants,adjuvants, Freund's
FREUND'S COMPLETE ADJUVANT, MODIFIED
FREUND'S COMPLETE ADJUVANT, MODIFIED, MYCOBACTERIUM BUTYRICUM
4-Amino-1-(beta-D-arabinofuranosyl)-5-fluoro-2(1H)-pyrimidinone | [Molecular Formula]
C9H12FN3O5 | [MDL Number]
MFCD00131105 |
| Hazard Information | Back Directory | [Uses]
Antigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion. | [Uses]
Immunization | [General Description]
1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension. | [Biochem/physiol Actions]
The mycobacteria in Complete Freund′s adjuvant attracts macrophages and other cells to the injection site which enhances the immune response. For this reason, Complete Freund′s Adjuvant is used for initial injections and Incomplete Freund′s Adjuvant for subsequent boosts. | [in vivo]
Complete Freund's adjuvant (CFA) induces experimental autoimmune myocarditis and neuropathic pain, with a relatively low plasma clearance rate (22%)[1][2][3][4].
Induction of experimental autoimmune myocarditis (EAM)[1] Background
Complete Freund's adjuvant (CFA) induces myocarditis by activating the immune system and enhancing the antigen-specific immune response.
Specific Modeling Methods
Mice: A/J, BALB/c, and IL6KO BALB/c ? male ? 6-8-week-old
Administration: 5mg/ml ? s.c. ? twice
Note
(1) On day 0, all mice additionally received 500 ng of pertussis toxin (i.p.)
(2) On days 0 and 7, mice received subcutaneous injections peptide emulsified in CFA supplemented with 5 mg/mL of Mycobacterium tuberculosis, strain H37Ra.
Modeling Indicators Histological analysis: Myocarditis severity was assessed by histology and relative heart weight.
Molecular changes: Test serum levels of total IgG, IgG1, IgG2a, IgG2b, IL-1b and IL6.
Correlated Product(s): Incomplete Freund's adjuvant (IFA) (HY-153808A); Complete Freund's adjuvant (CFA, 1 mg/ml) (HY-153808B) Opposite Product(s): /
Induction of Neuropathic Pain[2][3] Background
Complete Freund's adjuvant (CFA) releases inflammatory mediators TNF-α and IL-6 in body which can activate peripheral nerve endings and act on pain nerve endings, resulting in neuropathic pain[2][3].
Specific Modeling Methods
Mice: C57BL/6J ? male ? 8-week-old
Administration: 20 μl ? s.c. ? once
Note
(1) Inject Complete Freund’s Adjuvant (CFA) without dilution into the plantar area of the hind paw.
(2) Tests PWT and PWL for both hind paws corresponding to before and 24, 48, 72 h after CFA injection.
Modeling Indicators Behavior Observation: Detect paw withdrawal threshold (PWT) and paw withdrawal latency (PWL).
Correlated Product(s): Galantamine (HY-76299) Opposite Product(s): Mecamylamine (HY-B1395A) | Animal Model: | A/J, BALB/c, and IL6KO BALB/c male mice[1] | | Dosage: | | | Administration: | Subcutaneous Injectionon (sc); injected at days 0 and 7. EAM severity was analyzed by histology on day 21 (Repeated administration of CFA to animals can cause multiple injection-site effects, the most often observed are on site granulomas and pain) | | Result: | Increased the relative proportion of CD11b+F4/80?monocytes and CD11b+Gr1+ granulocytes.
Expanded monocytes in the heart and in the spleen and those monocytes in the spleen make less IL-10 as well as more IL-6. |
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