| Identification | Back Directory | [Name]
EC 2.7.1.40 | [CAS]
9001-59-6 | [Synonyms]
PK
EC 2.7.1.40
pyruvic kinase
PYRUVATE KINASE
PK （rabbit muscle)
Pyruvate Kinase M2
pyruvate phosphoferase
PYRUVATE KINASE TYPE I
PYRUVATE KINASE TYPE II
PYRUVATE KINASE TYPE III
PYRUVATE KINASE TYPE VIII
PHOSPHOENOL PYRUVATE KINASE
PyruvateKinaseExRabbitMuscle
Pyruvate Kinase, 50 units/mg
Pyruvate Kinase, 250 units/Mg
PYRUVATE KINASE, RABBIT MUSCLE
PYRUVATE O2-PHOSPHO TRANSFERASE
pyruvate kinase from rabbit muscle
Kinase (phosphorylating), pyruvate
Pyruvate Kinase froM MicroorganisM
phosphopyruvate transphos phorylase
ATP:PYRUVATE 2-O-PHOSPHOTRANSFERASE
ATP: PYRUVATE O2-PHOSPHOTRANSFERASE
Pyruvat kinase from rabbit, lyophil.
Pyruvate Kinase from rabbit liver
PYRUVATE KINASE TYPE X FROM CHICKEN &
PYRUVATE KINASE PRESTAINED FROM CHICKEN
ATP:pyruvate 2-O-phosphotransferase, PK
pyruvate kinase type X from chicken*muscle
pyruvate kinase type xii from rabbit*liver
pyruvate kinase type iii from rabbit*muscle
PYRUVATE KINASE TYPE II FROM RABBIT*MUSC LE
PYRUVATE KINASE TYPE VII FROM*RABBIT MUS CLE
Pyruvate Kinase preparation from rabbit muscle
PK(R.M.), ATP: pyruvate 2-o-phosphotransferase
pyruvate kinase type I crude*from rabbit muscle
PYRUVATE KINASE FROM BACILLUS STEAROTHERMOPHILUS
PYRUVATE KINASE, PRESTAINED FROM*CHICKEN MUSCLE
pyruvate kinase type viii from*bacillus stearothe
PYRUVATE KINASE FROM RABBIT MUSCLE, 100- 250 U/MG
PYRUVATE KINASE FROM PORCINE HEART (E.C. 2.7.1.40)
PYRUVATE KINASE TYPE VIII FROM*BACILLUS STEAROTHERM
Pyruvate Kinase preparation from canine muscle
Pyruvate kinase from rabbit muscle ca. 350 U/mg protein
pyruvate kinase from rabbit muscle cryst susp. ~350 U/mg
PYRUVATE KINASE FROM RABBITMUSCLE CA. 350 U/MG PROTEIN SUSP.
Pyruvate kinase (from Rabbit muscle), ammonium sulfate suspension
Pyruvate Kinase from rabbit muscle,ATP:pyruvate 2-O-phosphotransferase, PK
PYRUVATE KINASE (EC 2.7.1.40) FROM RABBIT MUSCLE HIGHLY PURIFIED SUSPENSION
Pyruvate Kinase from Bacillus stearothermophilus,ATP:pyruvate 2-O-phosphotransferase, PK
PYRUVATE KINASE (EC 2.7.1.40) FROM RABBIT MUSCLE LYOPHILIZED POWDER ESSENTIALLY SALT-FREE | [EINECS(EC#)]
232-616-2 | [Molecular Formula]
NULL | [MDL Number]
MFCD00081946 |
| Hazard Information | Back Directory | [Chemical Properties]
White powder | [Uses]
Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity. | [Uses]
Pyruvate kinase from rabbit muscle has been used in a structural study to understand the reaction mechanism of the final step in glycolysis. It has also been used in a study to investigate ATP-dependent phosphorylation of α-substituted carboxylic acids. | [Uses]
Pyruvate kinase has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein expression. The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase. It is involved in glycolysis and in gluconeogenesis. It has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. It is also used to study pyruvate kinase (PK) deficiency. | [General Description]
Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M1, and M2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M2 is localized in the nucleus of the cells. | [Biochem/physiol Actions]
Molecular Weight: 237 kDa?and exists as a tetramer of four equal subunits of molecular weight 57 kDa.Isoelectric Point: 7.6Optimal pH: ～7.5 Optimal Temperature: 25°C ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP. | [Purification Methods]
These are purified by (NH4)2SO4 fractionation and gel filtration, ion-exchange and affinity chromatography. [Garcia-Olalla & Garrido-Pertierra Biochem J 241 573 1987.] |
|
|