| Identification | Back Directory | [Name]
HUMAN FIBRINOGEN | [CAS]
9001-32-5 | [Synonyms]
C00393
factor 1
factor i
Parenogen
FIBRINOGEN
fibrinogens
Fibrinogen
HUMAN FIBRINOGEN
BOVINE FIBRINOGEN
FIBRINOGEN TYPE I
FIBRINOGEN, HUMAN
FIBRINOGEN, BOVINE
FIBRINOGEN, SALMON
FIBRINOGEN TYPE III
COAGULATION FACTOR I
Fibrinogen(Factor I)
FIBRINOGEN FRACTION 1
Fibrinogen froM huMan
human fibrinogen dried
FIBRINOGEN, PIG PLASMA
FIBRINOGEN, RAT PLASMA
FIBRINOGEN, DOG PLASMA
FIBRINOGEN, CAT PLASMA
from Bovine, fraction I
Native Bovine Fibrinogen
FIBRINOGEN, SHEEP PLASMA
FIBRINOGEN, HUMAN PLASMA
FIBRINOGEN, MOUSE PLASMA
FIBRINOGEN, BOVINE PLASMA
Fibrinogen,Rat60%Clottable
Fibrinogen,Dog60%Clottable
Fibrinogen,Pig60%Clottable
FIBRINOGEN FROM DOG PLASMA
HUMAN FIBRINOGEN USP/EP/BP
fibrinogen from cat plasma
FIBRINOGEN TYPE IV, BOVINE
Blood coagulation factor I
FIBRINOGEN FROM PIG PLASMA
FIBRINOGEN FROM RAT PLASMA
COAGULATION FACTOR I, HUMAN
FIBRINOGEN TYPE I-S, BOVINE
FIBRINOGEN AUS HUMANPLASMA*
FIBRINOGEN FROM MOUSE PLASMA
Fibrinogen,Horse60%Clottable
Fibrinogen,Human65%Clottable
fibrinogen from human plasma
FIBRINOGEN FROM SHEEP PLASMA
COAGULATION FACTOR I, BOVINE
FIBRINOGEN FROM BABOON PLASMA
FIBRINOGEN FROM BOVINE PLASMA
fibrinogen from canine plasma
fibrinogen from murine plasma
Fibrinogen,Bovine95%Clottable
Fibrinogen,Bovine75%Clottable
FIBRINOGEN(BOVINE),FRACTION I
fibrinogen from porcine plasma
FIBRINOGEN, FRACTION 1, BOVINE
FIBRINOGEN (BOVINE) (FRACTION 1)
FIBRINOGEN FROM GUINEA PIG PLASMA
Fibrinogen from Bovine, fraction I
Fibrinogen from bovine plasma,Factor I
FIBRINOGEN TYPE IV: FROM BOVINE PLASMA
FIBRINOGEN (FRACTION I) FROM CAT PLASMA
FIBRINOGEN, PLASMINOGEN-DEPLETED, HUMAN
Fibrinogen type III (from human plasma)
FIBRINOGEN TYPE I-S: FROM BOVINE PLASMA
Fibrinogen fraction 1 (from bovine plasma)
FIBRINOGEN, HUMAN TYPE I FROM HUMAN*PLAS MA
FIBRINOGEN, HUMAN TYPE III FROM HUMAN*PL ASMA
FIBRINOGEN, PLASMINOGEN-DEPLETED, HUMAN PLASMA
FIBRINOGEN, HUMAN (FRACTION I) FROM*HUMA N PLASMA | [EINECS(EC#)]
232-598-6 | [Molecular Formula]
NULL | [MDL Number]
MFCD00131060 | [MOL File]
9001-32-5.mol | [Molecular Weight]
182.172 |
| Chemical Properties | Back Directory | [Definition]
A sterile fraction of normal human blood plasma, dried from the frozen state. In solution, it has the property of being converted into insoluble fibrin when thrombin is added. It is an essential factor in the blood-clotting mechanism. | [Appearance]
White or grayish amorphous substance. | [storage temp. ]
−20°C
| [solubility ]
0.9% NaCl: soluble
| [form ]
powder
| [color ]
white
| [EPA Substance Registry System]
Fibrinogens (9001-32-5) |
| Hazard Information | Back Directory | [Chemical Properties]
White or grayish amorphous substance. | [Uses]
Diagnostic aid (vascular patency); radioactive agent. | [General Description]
Fibrinogen, or Factor I, is a blood protein that is involved in clotting and is converted to fibrin by thrombin. Fibrinogen has an approximate molecular weight of 340 kDa | [Biochem/physiol Actions]
Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source. | [Purification Methods]
A likely impurity is plasminogen. It is purified by glycine precipitation [Mosesson & Sherry Biochemistry 5 2829 1966] to obtain fractions 1-2, then further purified [Blomb.ck & Blomb.ck Arkiv Kemi 10 415 1956] and contaminating plasminogen is removed by passage through a lysine-Sepharose column. Such preparations are at least 95% clottable as determined by Mosesson and Sherry's method (above ref.) in which the OD280 is measured before and after clotting with 5 Units/mL of thrombin (> 3000U/mg). All fibrinogen preparations are treated with calf intestinal alkaline phosphatase to convert any fibrinogen peptide-AP to fibrinogen peptide-A by removing serine-bound phosphate. Solutions are then lyophilised and stored at -20o. [Higgins & Shafer J Biol Chem 256 12013 1981.] It is sparingly soluble in H2O. Aqueous solutions are viscous with isoelectric point at pH 5.5. It is readily denatured by heating above 56o or by chemical agents, e.g. salicylaldehyde, naphthoquinone sulfonates, ninhydrin or alloxan. [Edsall et al. J Am Chem Soc 69 2731 1947, Purification: Cama et al. Naturwissenschaften 48 574 1961, Lorand & Middlebrook Science 118 515 1953, cf. Fuller in Methods Enzymol 163 474 1988.] For plasminogen-deficient fibrinogen from blood plasma, the anticoagulated blood is centrifuged and the plasma is frozen and washed with saline solution. It is treated with charcoal, freeze-thawed and dialysed versus Tris/NaCl buffer. [Maxwell & Nikel Biochemical Preparations 12 16 1968.] |
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