| Identification | Back Directory | [Name]
Spectinomycin dihydrochloride pentahydrate | [CAS]
22189-32-8 | [Synonyms]
M 141
D00869
U-18409AE
spectogard
Trobicin (tn)
SPECTINOMYCIN HCL
SPECTINOMYCIN 2HCL
Spectinomycin,≥98%
Spectinomycin dihydrochL
Spectinomycin HCL USP/BP
SPECTINOMYCIN HYDROCHLORIDE
Dihydrochloride pentahydrate
Spectinomycin Hcl Sterile EP 5
ACTINOSPECTACIN DIHYDROCHLORIDE
m141dihydrochloridepentahydrate
Spectinomycin hydrochloride CRS
SPECTINOMYCIN.2HCLRESEARCH GRADE
Spectinomycin hydrochloride hydrate
SPECTINOMYCIN DIHYDROCHLORIDE PENTAH
Spectinomycin Hydrochloride (200 mg)
Spectinomycin hydrochloride (jp15/usp)
SOECTINOMYCINHYDROCHLORIDEPENTAHYDRATE
Spectinomycin dihydrochloride 5-hydrate
Spectinomycin for system suitability CRS
Togamycin dihydrochloride pentahydrate
SPECTINOMYCIN DIHYDROCHLORIDE PENT
SPECTINOMYCIN DIHYDROCHLORIDE HYDRATE, B
espectinomicinadihydrochloridepentahydrate
SPECTINOMYCIN DIHYDROCHLORIDE PENTAHYDRATE
SPECTINOMYCIN PENTAHYDRATE DIHYDROCHLORIDE
ACTINOSPECTACIN DIHYDROCHLORIDE PENTAHYDRATE
Spectinomycin hcl /Spectinomycin hydrochloride
SpectinoMycin-2HCl pentahydrate research grade
SPECTINOMYCIN DIHYDROCHLORIDE CELL*CULTU RE TESTED
Spectinomycin Dihydrochloride Pentahydrate Sterile
Spectinomycin dihydrochloride pentahydrate USP/EP/BP
SPECTINOMYCIN DIHYDROCHLORIDE GAMMA-*IRR ADIATED MOL
Spectinomycin dihydrochloride pentahydrate, 650 IU/mg
Spectinomycin Hydrochloride (200 mg)G0C310650ug/mg(ai)
Spectinomycin dihydrochloride pentahydrate, >=779 u/mg
Spectinomycin dihydrochloride pentahydrate, potency: ≥603 μg/mg
SPECTINOMYCIN DIHYDROCHLORIDE PENTAHYDRATE from Streptomyces sp.
SPECTINOMYCIN, DIHYDROCHLORIDE, PENTAHYDRATE, STREPTOMYCES SPECIES
4H-Pyrano2,3-b1,4benzodioxin-4-one, decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-, dihydrochloride, pentahydrate, (2R,4aR,5aR,6S,7S,8R,9S,9aR,10aS)-
(2R,4aR,5aR,6S,7S,8R,9S,9aR,10aS)-4a,7,9-Trihydroxy-2-methyl-6,8-bis(methylamino)decahydro-4H-benzo[b]pyrano[2,3-e][1,4]dioxin-4-one dihydrochloride pentahydrate
4H-Pyrano[2,3-b][1,4]benzodioxin-4-one,decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-, hydrochloride,hydrate (1:2:5), (2R,4aR,5aR,6S,7S,8R,9S,9aR,10aS)-
2alpha,4abeta,5abeta,6beta,7beta,8beta,9alpha,9aalpha,10abeta)]-Decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis-(methylamino)-4H-pyrano[2,3-b][1,4]benzodioxin-4-one dihydrochloride pentahydrate | [EINECS(EC#)]
244-554-3 | [Molecular Formula]
C14H24N2O7.2HCl.5H2O | [MDL Number]
MFCD00070403 | [MOL File]
22189-32-8.mol | [Molecular Weight]
495.35 |
| Chemical Properties | Back Directory | [Appearance]
White or almost white, slightly hygroscopic powder. | [Melting point ]
205-207° (dec) | [alpha ]
D +14.8° (c = 0.42 in water) | [storage temp. ]
2-8°C
| [solubility ]
H2O: 50 mg/mL, clear, faintly yellow
| [form ]
powder
| [color ]
white to off-white | [Water Solubility ]
Soluble in water.Soluble in water, methanol, dimethyl sulfoxide, ethanol and dimethyl formamide. | [Merck ]
14,8739 | [BRN ]
5684150 | [InChIKey]
DCHJOVNPPSBWHK-UXXUFHFZSA-N | [CAS DataBase Reference]
22189-32-8 |
| Hazard Information | Back Directory | [Chemical Properties]
White or almost white, slightly hygroscopic powder. | [Uses]
Aminocyclotol antibacterial. | [Definition]
ChEBI: A hydrate that is the pentahydrate form of spectinomycin hydrochloride. An antibiotic that is active against gram-negative bacteria and used to treat gonorrhea. | [Uses]
Spectinomycin is an aminocyclitol antibiotic produced by S. spectabilis that is active against Gram-negative and Gram-positive bacteria. Spectinomycin inhibits protein synthesis by binding to the 30S ribosomal subunit and interfering with peptidyl tRNA translocation. Mutations in the gene for ribosomal protein S5 prevents binding of spectinomycin and contributes to bacterial resistance. | [Originator]
Trobicin,Upjohn,US,1971 | [Manufacturing Process]
A lyophilized culture of Streptomyces spectabilis, NRRL 2792, was used to
seed the following sterile agar medium on tubed slants:Tryptone 5
K2HPO4 0.5
NaCl 0.5
FeSO4 0.1
Agar 20
Deionized water to make 1 liter
The slants were incubated for 7 days at 30°C, after which time sporulation
was complete. The spores from the agar slants were used, in an aqueous
suspension, to inoculate 100 ml of preseed medium in a 500 ml Erlenmeyer
flask. The sterile preseed medium consisted of:
Grams
Dried whole yeast 10
Glucose 10
Pancreatic digest of 5
casein (N-Z-Amine B)
Tap water to make 1 liter adjusted to pH 7.2 before sterilizing
The seed flash was incubated for 24 hours at 32°C on a reciprocating shaker
after which it was used as an inoculum for a 20 liter seed fermenter in the
amount of approximately 5%. the 20 liter seed fermenter contained a sterile
medium consisting of:
Grams
Glucose 15
Cornstarch 25
Distiller's solubles 15
Brewer's yeast 10
Corn steep liquor 20
Tap water to make 1 liter adjusted to pH 7.2 before sterilizing
The 20 liter seed fermenter was incubated for 24 hours at 32°C and aerated
at the rate of 6 standard liters or about 0.2 standard cubic feet of air per
minute and agitated with a sweep stirrer. The 20 liter seed fermenter was
used to inoculate 250 liters of the same medium in a 100 gallon fermentation
tank. 1,200 ml of lard oil were added during the fermentation to control
foaming. The tank was agitated with a propeller and aerated at the rate of 75
standard liters of air per minute. After 96 hours of fermentation the beer
assayed 500 mcg/ml (18.3 mcg/mg on a dry basis) of actinospectacin.
Actinospectacin is assayed by its activity against Klebsiella pneumoniae by
standard agar diffusion procedure and based on crystalline actinospectacin
sulfate according to US Patent 3,234,092. | [Therapeutic Function]
Antibacterial | [General Description]
A broad spectrum aminoglycoside antibiotic that contains two glucose moieties. Footprint studies indicate that spectinomycin exerts regional effects on ribosomal structure. A resistance gene (aph) which encodes a spectinomycin has been cloned from Legionella pneumophila (disease agent for Legionnaire’s disease) kinase and expressed in E. coli. Potency: ≥500 μg/mg. | [Biological Activity]
spectinomycin is an aminocyclitol antibiotic produced by s. spectabilis with activity against gram-negative bacterial species. spectinomycin has been used for the treatment of gonorrhea, where it inhibits microbial protein synthesis by binding to the 30s ribosomal subunit, protects the n‐7 position of e. coli 16s rrna residue g1064 from methylation by dimethyl sulfate. binding of spectinomycin at this location is thought to stabilize helix 34, inhibiting the binding of elongation factor g, thereby blocking translocation of peptidyl‐trnas from the ribosomal a site to the p site [1].spectinomycin selectively inhibited protein synthesis in cells and in extracts of e. coli. mutations to high-level resistance to this antibiotic map close to the streptomycin locus, and the site of action of spectinomycin, like that of streptomycin, is the 30s ribosomal subunit, as shown by experiments with reconstituted 70s ribosomes containing subunits from sensitive and from resistant ribosomes [2]. | [Biochem/physiol Actions]
Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA). Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria. | [storage]
Store at 2-8°C | [References]
[1] zimmerman j m, maher l j. in vivo selection of spectinomycin‐binding rnas[j]. nucleic acids research, 2002, 30(24): 5425-5435.
[2] davies j, anderson p, davis b d. inhibition of protein synthesis by spectinomycin[j]. science, 1965, 149(3688): 1096-1098. |
| Safety Data | Back Directory | [Safety Statements ]
24/25 | [WGK Germany ]
2
| [RTECS ]
WG7400000
| [F ]
10 | [HS Code ]
29419090 | [Toxicity]
mouse,LD50,intramuscular,> 5gm/kg (5000mg/kg),BEHAVIORAL: SOMNOLENCE (GENERAL DEPRESSED ACTIVITY),Japanese Journal of Antibiotics. Vol. 29, Pg. 43, 1976. |
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