| Identification | Back Directory | [Name]
Hemin | [CAS]
16009-13-5 | [Synonyms]
HEMIN
HAEMIN
Hemin,99%
Hemin, USP
CHLOROHEMIN
Hemin, 98+%
EQUINE HEMIN
HEMIN PORCINE
PROTOHEMIN IX
HEMIN, BOVINE
Heme chloride
Chlorhaematin
Protoferriheme
HEMIN CHLORIDE
HEMIN ULTRA-PURE
Hematin chloride
Hemin from Bovine
FerriheMe chloride
HEMIN, BOVINE 98+%
Haemin,Crystalline
ChloroprotoferriheM
Protohemin chloride
Hemin, 99%, porcine
Teichmann's crystals
Chloroprotoferriheme
HEMIN,BOVINE,CRYSTAL
Hemin (from porcine)
HEMIN CHLORIDE BOVINE
HEMATIN HYDROCHLORIDE
HeMin (porcine), 98+%
HeMatine hydrochloride
HEMIN CHLORIDE PORCINE
Hemin from porcine blood
CHLOROFERRIPROTOPORPHYRIN | [EINECS(EC#)]
240-140-1 | [Molecular Formula]
C34H32ClFeN4O4 | [MDL Number]
MFCD00010726 | [MOL File]
16009-13-5.mol | [Molecular Weight]
651.94 |
| Chemical Properties | Back Directory | [Appearance]
Dark purple crystalline powder | [Melting point ]
300 °C
| [storage temp. ]
2-8°C
| [solubility ]
1.4 M NaOH: soluble25mg/mL | [form ]
crystal | [color ]
black | [Stability:]
Stable. Incompatible with strong oxidizing agents. Combustible. | [Water Solubility ]
practically insoluble | [Merck ]
14,4644 | [BRN ]
5229914 | [Exposure limits]
ACGIH: TWA 1 mg/m3
NIOSH: TWA 1 mg/m3 | [InChIKey]
BTIJJDXEELBZFS-HXFTUNQESA-K | [SMILES]
[Cl-][Fe+3]123[N-]4C5C(C)=C(C=C)C4=CC4C(C)=C(C=C)C(=CC6=C(C)C(CCC(=O)[O-])=C(C=C7C(CCC(=O)[O-])=C(C)C(C=5)=N17)[N-]26)N3=4.[H+] | [Uses]
Identification of blood stains, biochemical research, complexing agent. | [EPA Substance Registry System]
Ferrate(2-), chloro[7,12-diethenyl-3,8, 13,17-tetramethyl-21H,23H- porphine-2,18-dipropanoato( 4-)-.kappa.N21,.kappa.N22,. kappa.N23,.kappa.N24]-, dihydrogen, (SP-5-13)-(16009-13-5) |
| Hazard Information | Back Directory | [Chemical Properties]
Dark purple crystalline powder | [Description]
Hemin(16009-13-5) is a metalloporphyrin compound with a relatively simple structure. It is formed by the complexation of a molecule of ferrous iron and protoporphyrin. It is an in vitro group substitute of natural heme and its chemical properties are similar to that of heme. Heme, an essential small molecule in living organisms, can be found abundantly in various organisms including animals, plants, microorganisms, and algae. | [Application]
Hemin is used for the following applications:
To study bacterial strains and growth conditions
Used in short-term colony assays (the culture medium consists of hemin along with other components)
Cell transfections
It may be used in β-hematin formation assay for analyzing the inhibitory activity of MMV (Medicines for Malaria Venture) Malaria Box compounds against the formation of β-hematin. | [General Description]
Heme is a small molecule present either free or bound to hemoglobin in the bloodstream of mammals. This is an alternative source of iron within the host that contains a porphyrin ring containing a Fe2+ ion called hemin. | [Biochem/physiol Actions]
Hemin, the oxidized version of heme, is an iron-containing prosthetic group for a diverse group of proteins. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Free hemin levels may be upregulated in various pathological conditions and can contribute to various inflammatory conditions including vascular disorders, renal failure, and immune-mediated disorders.Hemin may be used to study the expression and represson of heme oxygenase 1 (HO-1) as well as the activity of HO-1. Hematin is the "X factor" required to grown Haemophilus influenzae. | [storage]
4°C, protect from light, stored under nitrogen | [Purification Methods]
Hemin is purified by recrystallisation from AcOH. Also, hemin (5g) is shaken in pyridine (25mL) till it dissolves, then CHCl3 (40mL) is added, the container is stoppered and shaken for 5minutes (releasing the stopper occasionally). The solution is filtered under slight suction, and the flask and filter are washed with a little CHCl3 (15mL). During this period, AcOH (300mL) is heated to boiling, and saturated aqueous NaCl (5mL) and conc HCl (4mL) are added. The CHCl3 filtrate is poured in a steady stream, with stirring, into the hot AcOH mixture and set aside for 12hours. The crystals are filtered off, washed with 50% aqueous AcOH (50mL), H2O (100mL), EtOH (25mL), Et2O and dried in air. [Fischer Org Synth Coll Vol III 442 1955, Beilstein 26 III/IV 3048.] |
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