| Identification | Back Directory | [Name]
BIOTIN-4-FLUORESCEIN | [CAS]
1032732-74-3 | [Synonyms]
5-FITC-4-Biotin-4
Biotin Impurity 30
BIOTIN-4-FLUORESCEIN
Biotin-4-fluorescein *CAS 1032732-74-3*
(3aS,4S,6aR)-N-[2-[[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]amino]ethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
1H-Thieno[3,4-d]imidazole-4-pentanamide, N-[2-[[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]amino]ethyl]hexahydro-2-oxo-, (3aS,4S,6aR)- | [Molecular Formula]
C33H32N4O8S | [MDL Number]
MFCD03427760 | [MOL File]
1032732-74-3.mol | [Molecular Weight]
644.69 |
| Chemical Properties | Back Directory | [Boiling point ]
1038.0±65.0 °C(Predicted) | [density ]
1.55±0.1 g/cm3(Predicted) | [storage temp. ]
room temp | [form ]
powder | [pka]
9.33±0.20(Predicted) | [color ]
light orange to very dark orange
light yellow to very dark yellow | [ε(extinction coefficient)]
≥70 at 491-497nm in phosphate buffer at 0.05M |
| Hazard Information | Back Directory | [Uses]
Biotin-4-Fluorescein is used to quantitate biotin binding sites. Biotin-4-Fluorescein is a reagent that binds to avidin more readily than conventional fluorescein biotin. | [Biological Activity]
Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenchingwhich is used for measuring the concentration of (strept)avidin. |
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